Effect of SEPT7 on the malignant phenotype of transplanted glioma in nude mice
10.3321/j.issn:0253-3766.2008.01.002
- VernacularTitle:隔蛋白7逆转裸鼠移植胶质瘤的恶性表型
- Author:
Zhi-Fan JIA
1
;
Pei-Yu PU
;
Chun-Sheng KANG
;
Guang-Xiu WANG
;
Zhi-Yong ZHANG
;
Ming-Zhe QIU
;
Qiang HUANG
Author Information
1. 天津市神经病学研究所
- Keywords:
SEPT7;
Glioma model;
Cyclin;
Proliferation;
Invasion
- From:
Chinese Journal of Oncology
2008;30(1):3-7
- CountryChina
- Language:Chinese
-
Abstract:
Objective To study the effect of SEPT7 on glioma cell growth, cell cycle progression, invasion and apoptosis in vivo. Methods Nude mice with subcutaneously transplanted human glioma TJ905, in which SEPT7 is absent, were divided into three groups: SEPT7 treatment group, empty vector treatment group, and control group (PBS). The expression of SEPTT, PCNA, GFAP, proteins involved in the regulation of G1/S phase progression (cylcin D1, cylcin E, CDK2, CDK4, p21, p16), as well invasion-related factors (MMP2, MMPg) and apoptosis-related factors ( bcl-2, caspase-3) were determined by immunohistochemistry in the glioma samples taken from the mice in the three groups. TUNEL method was used to detect the apoptotic index of tumors. Results During the 4 weeks-observation period, the growth rate of gliomas in the nude mice in SEPT7 group was significantly slowed down and the tumor volume was much smaller than those in control and empty vector groups. In the glioma treated with SEPTT, the expressions of SEPTT, p21., p16 and caspase-3 were significantly upregulated, the expressions of PCNA, MMP-2, MMP-9, cyclin D1, cyclin E, CDK2, CDK4 and bcl-2 were reduced, and GFAP was positive. Increase of apoptotic cells was observed by TUNEL. Conclusion SEPT7 can reverse the malignant phenotype of glioma cells by inhibition of cell growth and invasion, and induction of apoptosis, and SEPT7 is supposed preliminary to be a tumor suppressor gene.
