Immunoelectron microscopic analysis of P-glycoprotein,p53, and Bcl-2 proteins expressions in lung cancer
- VernacularTitle:免疫电镜方法研究P-gp、p53蛋白、Bcl-2蛋白在肺癌组织中的表达
- Author:
Xiaoming HUANG
1
;
Longyun LI
;
Zhenquan GUO
Author Information
1. Peking Union Medical College Hospital Chinese Academy of Medical Sciences
- From:
Chinese Journal of Oncology
2001;23(1):53-56
- CountryChina
- Language:Chinese
-
Abstract:
Objective To determine the ultrastructural localization of P-gp, p53 protein, and Bcl-2 protein in lung cancer cells,their relation to multidrug resistance and possible mechanisms of action. Methods Expression of P-gp, p53, and Bcl-2 proteins was examined in 8 NSCLC surgical specimens and 7 SCLC bronchoscopically biopsied specimens using postembedding PAG immunolabelling technique for electron microscopy. Results P-gp was detected on the cell membrane and the periphery of endoplasmic reticulum(ER). P53 protein was observed not only in the nuclei associated with heterochromatin but also in the cytosol. Bcl-2 protein immunoreactivity was associated with mitochondria and ER. P-gp, p53, and Bcl-2 were detected in 5(33%), 9(60%), and 4(26.7%) out of the 15 samples examined，respectively. In 8 normal lung tissues, these three proteins were detected. Of 5 P-gp positive samples 4 were NSCLC, only 1 was SCLC after chemotherapy. Conclusion P-gp, p53, and Bcl-2 proteins are detectable immuno-electron microscopically in lung cancer cells. No correlation in expression existed between of p53 and P-gp, nor did that between p53 and Bcl-2. The plasma membrane localization of P-gp supports its action as a transmembrane drug efflux pump. P-gp may play a role in MDR in lung cancer.
