Determination of acetone in urine by headspace gas chromatography-mass spectrometry
10.3760/cma.j.cn121094-20220815-00401
- VernacularTitle:尿中丙酮含量测定的顶空气相色谱-质谱法
- Author:
Xiangjuan MENG
1
;
Xiaomei ZHANG
;
Yue HU
;
Xiaodong LIU
Author Information
1. 北京市化工职业病防治院中心实验室，北京 100093
- Keywords:
Urine;
Acetone;
Headspace;
Gas chromatography-mass spectrometry
- From:
Chinese Journal of Industrial Hygiene and Occupational Diseases
2023;41(11):849-851
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To establish a method for determination of acetone in urine by headspace gas chromatography-mass spectrometry.Methods:From March to June 2021, the 3.0 ml urine sample was placed in a headspace bottle with 4.0 g of anhydrous sodium sulfate and sealed. Equilibration time was 30 min at 85 ℃. The separation was carried out on a DB-5MS column. The urine sample was detected by mass spectrometry and quantified by external standard method.Results:The method for the determination of acetone in urine by headspace gas chromatography-mass spectrometry had good linearity in the range of 51.2-1024.0 μg/L, and the correlation coefficient was 0.9995. The detection limit and the lower limit of quantification of acetone were 16.4 μg/L and 54.6 μg/L. The average recoveries of samples ranged from 94.9% to 96.8%. The intra-assay precision and inter-assay precision were both less than 10%. Samples can be stored at least 7 d at 4 ℃ or -20 ℃.Conclusion:This method has simple sample preparation and high sensitivity. It can be used for monitoring and evaluation of urinary acetone in the general population and occupationally exposed populations.
