Determination of six arsenic species in urine by high-performance liquid chromatography-inductively coupled plasma-mass spectrometr
10.3760/cma.j.cn121094-20211231-00646
- VernacularTitle:尿液中6种砷形态化合物测定的高效液相色谱-电感耦合等离子体质谱法
- Author:
Yue SHEN
1
;
Daoyuan SUN
;
Zhen MIN
Author Information
1. 同济大学附属上海市肺科医院中毒科，上海 200433
- Keywords:
Urine;
Arsenic speciation;
High-performance liquid chromatographyinductively coupled plasma-mass spectrometry
- From:
Chinese Journal of Industrial Hygiene and Occupational Diseases
2022;40(12):952-955
- CountryChina
- Language:Chinese
-
Abstract:
Objective:Establishing an analysis method for the detection of six arsenic compounds trivalent arsenic, pentavalent arsenic, methyl arsenic, dimethyl arsenic, arsenical choline and arsenical betaine in urine by high-performance liquid chromatography-inductively coupled plasma-mass spectrometry (HPLC-ICP-MS) and providing a theoretical basis for health assessment of arsenic poisoning patients.Methods:HPLC-ICP-MS was used to detect six arsenic compounds with the hamilton PRP-X100 column and aqueous ammonium phosphate in a gradient elution as mobile phase.Results:There was a good linear relationship in the selected range of six arsenic compounds evidenced by a linear correlation coefficient greater than 0.999. The detection limit of 6 arsenic compounds was 0.46~0.57 μg/L, and the minimum quantitative concentration were 1.52~1.91 μg/L. The recovery rates of six arsenic compounds were 96.5%~106.4%, The within-run and between-run relative standard deviation was 2.9%~6.2% and 3.8%~8.7%.Conclusion:The established method with high accuracy and precision is suitable for detecting arsenic levels in urine of occupational arsenic exposure population.
