Effects of Nd 2O 3 exposure of rare earth particles on C57 BL/6J male mice sex hormone secretion and CYP11A1/PLZF/STRA8 protein expression
10.3760/cma.j.cn121094-20210817-00401
- VernacularTitle:稀土颗粒物Nd 2O 3暴露对C57 BL/6J雄性小鼠性激素分泌及CYP11A1/PLZF/STRA8蛋白表达的影响
- Author:
Haiyan SONG
1
;
Ning BU
;
Yanrong GAO
;
Yuhang ZHAO
;
Xuemin SHI
;
Suhua WANG
Author Information
1. 内蒙古科技大学包头医学院劳动卫生与环境卫生学教研室，包头 014040
- Keywords:
Nd 2O 3;
Sperm;
Testis;
Sex hormones;
Lnteinizing hormone;
Follicle stimulating hormone;
Testosterone
- From:
Chinese Journal of Industrial Hygiene and Occupational Diseases
2022;40(12):881-887
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To explore the effects of Nd 2O 3 exposure to rare earth particles on the secretion of sex hormones, cytochrome P450 family member 11A1 (CYP11A1) , spermatogenesis markers promyelocytic leukemia zinc finger protein (PLZF) and retinoic acid stimulating gene 8 (STRA8) protein in C57 BL/6J male mice. Methods:In March 2021, Forty-eight male C57 BL/6J mice aged 6-8 weeks divided into control group and Nd 2O 3 exposure low, medium and high dose groups (exposing doses of 62.5, 125.0, 250.0 mg/ml Nd 2O 3) , 12 per group. The mice in the Nd 2O 3 groups were perfused with different doses of Nd 2O 3 suspension by a one-time non-exposing tracheal instillation method, and the control group was perfused with an equal volume of normal saline, with a volume of 0.1 ml, to establish a mouse reproductive function injury model. After 28 days of exposure, the mice's body weight, testes and epididymis were weighed, and the organ coefficients were calculated; the two epididymis were taken to make a sperm suspension to determine the sperm count, survival rate, and deformity rate; inductively coupled plasma mass spectrometry (ICP-MS) method was used to detect the content of Nd in mouse testis tissue; HE staining was used to detect testicular tissue pathological changes and quantitative analysis; enzyme-linked immunosorbent assay (ELISA) method was used to detect serum luteinizing hormone (LH) and follicle stimulating hormone (FSH) and testosterone (T) content; western blot was used to detect the protein levels of CYP11A1, PLZF and STRA8 in testicular tissues. Results:Compared with the control group, with the increase of the exposure dose, the Nd content in the testis of the mice showed an increasing trend, the sperm survival rate and LH showed a decreasing trend, and the sperm deformity rate showed an increasing trend ( P<0.05) ; Pathological showed that the number of sperm in the seminiferous tubules of the testicular tissue in the Nd 2O 3 medium and high dose groups was significantly reduced, and the germinal epithelial disintegration, intraepithelial vacuolization, and exfoliation of spermatogenic cells and supporting cells occurred; The height of germinal epithelium was significantly reduced, and the percentage of damaged seminiferous tubules showed an increasing trend ( P<0.05) ; FSH and T levels in serum in the middle and high dose groups of Nd 2O 3, and CYP11A1, PLZF and STRA8 proteins in testicular tissues showed a downward trend with increasing dose ( P<0.05) . Conclusion:The rare earth particulate Nd 2O 3 may interfere with the expression of CYP11A1, PLZF and STRA8 protein, thereby causing the disorder of sex hormone secretion in the body, the maintenance of spermatogonia and the obstruction of the process of meiosis, causing reproductive function damage.
