Distribution and metabolism of toxicants in rats with phenol burn
10.3760/cma.j.cn121094-20200710-00386
- VernacularTitle:苯酚烧伤大鼠的体内毒物分布及代谢
- Author:
Liqun TENG
1
;
Xin LIAO
;
Wei LI
Author Information
1. 201508 上海，复旦大学附属金山医院核化学伤害应急救治中心烧伤科
- Keywords:
Phenol;
Burns;
Rats;
Chromatography, high-performance liquid;
Toxicokinetics
- From:
Chinese Journal of Industrial Hygiene and Occupational Diseases
2021;39(11):859-861
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To study the distribution and metabolism of toxicants in rats after phenol burn.Methods:In February 2019, SPF-grade healthy SD male rats were transdermally exposed to 6 mg/kg phenol to create a 5% body surface burn model of rats. High performance liquid chromatography was used to determine phenol content in rat plasma and kidney tissues after 0.25, 0.75, 2, 4, 8, 16, and 32 h, respectively. The kinetic parameters of phenol were calculated by DAS 2.0 software, and the kidney targeting of phenol was evaluated.Results:The area under the blood concentration-time curve at 0-8 h (AUC 0-8) of the rat after phenol burn was (28.741±6.485) μg/ml·h, and the area under the blood concentration-time curve from 0 to infinite time (AUC 0-∞) was (30.354±6.424) μg/ml·h, half-life ( t1/2) was (2.111±0.632) h, peak concentration ( Cmax) was (16.287±4.870) μg/ml, mean residence time (MRT) was (1.854±0.148) h. The target efficiency (DTE) of rat kidney was 2.91. Conclusion:Phenol burn rats have fast percutaneous absorption, rapid elimination of phenol, and have high clearance rate, short MRT, and weak substance accumulation. Phenol has relatively obvious selectivity to the kidneys.
