Liquid chromatography-tandem mass spectrometry method for the determination of paraquat and diquat in plasma and urine
10.3760/cma.j.cn121094-20210125-00048
- VernacularTitle:血浆和尿液中百草枯和敌草快测定的固相萃取-液相色谱-串联质谱法
- Author:
Xiang GUO
1
;
Tiandi LI
;
Dongchao TIAN
;
Changhong MA
;
Yiran LIN
;
Jianpei YUN
Author Information
1. 518020 深圳市职业病防治院
- Keywords:
Paraquat;
Diquat;
LC-MS/MS;
Plasma;
Urine
- From:
Chinese Journal of Industrial Hygiene and Occupational Diseases
2021;39(8):612-616
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To establish a LC-MS/MS method for determination of paraquat and diquat in plasma and urine samples.Methods:Plasma is precipitated by acetonitrile then diluent with phosphate buffer (pH=7) , urine is diluent with phosphate buffer (pH=7) , then diluent samples extracted with Oasis WCX solid-phase extraction column. Samples were analyzed using LC-MS/MS in multiple reaction monitoring (MRM) mode. The analytical column was XBridge?BEH-HILIC (100 mm×2.1 mm×2.5 μm) and the mobile phase were 100 mmol ammonium formate add 0.5% formic acid and acetonitrile. Paraquat was quantified by internal standard method and diquat by external standard method.Results:The calibration curves of paraquat and diquat were linear in the concentration range of 10.0~120.0 μg/L, the correlation coefficient (r) were 0.9985~0.9994. The limit of detection of paraquat in plasma and urine were 1.98 μg/L and 1.00 μg/L, respectively, the recovery rate were 100.2%~107.3%, the RSD were 1.6%~3.3%. The limit of detection of diquat in plasma and urine were 1.80 μg/L and 2.77 μg/L, respectively, the recovery rate were 85.3%~93.1%, the RSD were 1.8%~5.5%. Conclusion:This method is sensitive and accurate, and can simultaneously determine paraquat and diquat in plasma and urine.
