Determination of five phenolic compounds in workplace air by composite sampling tube-high performance liquid chromatography
10.3760/cma.j.cn121094-20200428-00229
- VernacularTitle:工作场所空气中五种酚类化合物测定的复合采样管采集-高效液相色谱法
- Author:
Weili SONG
1
;
Zheng ZHANG
;
Jinfeng JIANG
;
Li YANG
;
Yapan LIU
Author Information
1. 430015 武汉市职业病防治院卫生检验科
- Keywords:
Chromatography, high pressure liquid;
Workplace;
Air;
Composite sampling tube;
Phenolic compounds
- From:
Chinese Journal of Industrial Hygiene and Occupational Diseases
2021;39(8):605-609
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To develop a method for simultaneous determination of Dydroquinone, Resorcinol, Pyrocatechol, 4-Nitrophenol and 2, 4-Dinitrophenol in workplace air by high performance liquid chromatography.Methods:Air samples were collected by composite tube (front end glass fiber filter membrane, back section silica gel) , 10% methanol was desorbed, separated by C18 chromatographic column, detected by photo-diode array (PDA) detector, and quantitatively determined by external standard method at the wave-length of 230 nm.Results:The linear relationship of 5 phenolic compounds was good ( r>0.999) . The detection limit of glass fiber filtration membrane and silica gel adsorbent were 0.13-0.41 g/ml and 0.16-1.04 g/ml respectively. The quantitative limit of glass fiber filtration membrane was 0.44-1.36 g/ml, and the silica gel adsorbent was 0.52-3.46 g/ml. The average desorption efficiency of glass fiber membrane and silica gel adsorbent were 97.5%-100.1% and 86.9%-100.3%, respectively. In and between batches, the precision glass fiber filtration membrane was 0.71%-4.88%, 0.91%-4.82%, silica gel adsorbent was 0.47%-4.62%, 0.76%-5.52%. Samples can be stored for at least 30 days at -20 ℃. The possible co-existing interferences of aniline, phenol, p-nitrochlorobenzene, o-nitrophenol and trinitrophenyl did not interfere with the determination. Conclusion:The sensitivity, precision, accuracy and linear range of this method all meet the requirements of the specification. The collection and preservation of samples can also meet the requirements of the limits. It is suitable for the simultaneous determination of hydroquinone, resorcinol, hydroquinone, hydroquinone, hydroquinone, p-nitrophenol and 2, 4-dinitrophenol in the air of the workplace.
