Determination of Methoxyacetic acid in urine by pre-column derivatization-liquid-liquid microextraction coupled with gas chromatography
10.3760/cma.j.cn121094-20200603-00317
- VernacularTitle:尿中甲氧基乙酸测定的柱前衍生-液液微萃取-气相色谱法
- Author:
Yingying GU
1
;
Yong MEI
;
Wanting SU
;
Jun HAN
Author Information
1. 430065 武汉科技大学医学院公共卫生学院
- Keywords:
Gas chromatography;
Methoxyacetic acid;
Liquid-Liquid extraction;
Pre-column derivatization-liquid-liquid microextraction;
Urine
- From:
Chinese Journal of Industrial Hygiene and Occupational Diseases
2021;39(8):602-605
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To establish a method for determining methoxyacetic acid in urine by pre-column derivatization-liquid-liquid microextraction coupled with gas chromatography (GC) .Methods:Phosphate buffer solution, tert-butoxyacetic acid (internal standard) and pentafluorobenzyl bromide (derivative) were added to the urine sample. After derived in a water bath at 90 ℃ for 40 min, the mixture was cooled and filtered, then the dichloromethane was used as an extractant. After being shaken and centrifuged, the lower organic phase was sucked and injected into a gas chromatograph, separated by a DB-5 capillary column, and detected by an ECD detector.Results:The linear range of the method was 0.6~60.0 mg/L with the correlation coefficients ( r) above 0.999. The average recovery was76.6%~110.7%, the inter-day precision was 8.00%~8.82%, and the detection limit was 0.13 mg/L. Conclusion:The method was founded to be high sensitivity, low organic reagent usage and green. So it is suitable for the detection of methoxyacetic acid in urine of occupational exposure to ethylene glycol monomethyl ether.
