Study on malignant transformation of MeT-5A cells induced by chrysotile
10.3760/cma.j.cn.121094-20200831-00508
- VernacularTitle:温石棉诱导MeT-5A细胞恶性转化的研究
- Author:
Fangfang ZHANG
1
;
Xiuyuan YUAN
;
Yanan GAO
;
Min ZHANG
;
Min YU
;
Man YU
;
Yun XIAO
;
Li JU
;
Yali HUANG
;
Xing ZHANG
;
Lijin ZHU
Author Information
1. 310013 杭州医学院（浙江省医学科学院）公共卫生学院
- Keywords:
Cell transformation;
Glycolysis;
Chrysotile;
Cytotoxicity;
Tumor
- From:
Chinese Journal of Industrial Hygiene and Occupational Diseases
2021;39(2):105-109
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To study the cytotoxicity and malignant transformation ability of chrysotile on MeT-5A cells.Methods:In June 2016, lactate dehydrogenase (LDH) method was used to detect the cytotoxicity of chrysotile to MeT-5A cells. MeT-5A cells were treated with 5 μg/cm 2 chrysotile intermittently for 24 h a time, once a week and a total of 28 times. After the cells showed anchorage independent growth, the cell features of malignant transformation were identified by colony forming frequency in soft agar, and the soft agar colony formation rates were calculated. The activities of key speed limiting enzymes of glycolysis metabolism including hexokinase (HK) , phosphofructokinase (PFK) and pyruvate kinase (PK) were determined by UV colorimetry. Results:Chrysotile was cytotoxic to MeT-5A cells in a concentration-dependent decline. Compared with the control group, the relative survival rates of MeT-5A cells were significantly decreased after exposed to chrysotile at 10, 20, 40 and 80 μg/cm 2 ( P<0.05) . After 28 times of exposure, the growth rate of the cells in chrysotile transformed MeT-5A cells was accelerated, the arrangement was disordered, the contact inhibition was lost, and the double layer growth appeared, which could grow on soft agar. The colony forming rate of the chrysotile transformed MeT-5A cells was 18.33‰±2.49‰. Compared with the control group (0) , the difference was statistically significant ( P<0.01) . The activities of glycolysis related kinase including PK [ (19.51±1.52) U/L], PFK[ (0.12±0.02) U/10 4 cell] and HK[ (0.26±0.01) U/10 4 cell] were increased in the chrysotile transformed MeT-5A cells compared with control group [ (25.00±1.04) U/L、(0.15±0.01) U/10 4 cell and (0.33±0.01) U/10 4 cell] ( P<0.01) . Conclusion:Chrysotile can induce malignant transformation of MeT-5A cells and increase the activities of glycolysis related kinases including PK, PFK and HK.
