Determination of metabolites of styrene in urine by dispersive liquid-liquid microextraction coupled with high performance liquid chromatography
10.3760/cma.j.cn121094-20191010-00469
- VernacularTitle:尿中苯乙烯代谢产物测定的分散液液微萃取-高效液相色谱法
- Author:
Yingying GU
1
;
Yong MEI
;
Menghan NIE
;
Xuguang SHENG
;
Ruodan FANG
;
Wanting SU
;
Jun HAN
Author Information
1. 430065 武汉科技大学医学院公共卫生学院；430065 武汉科技大学资源与环境工程学院；430065 武汉科技大学，职业危害识别与控制湖北省重点实验室
- Keywords:
Mandelic acid;
Phenylglyoxylic acid;
Dispersive liquid-liquid microextraction;
High performance liquid chromatography;
Urine
- From:
Chinese Journal of Industrial Hygiene and Occupational Diseases
2020;38(9):689-692
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To establish a method for the determination of mandelic acid and phenylglyoxylic acid in the urine of styrene by dispersive liquid-liquid microextraction-high coupled with high performance liquid chromatography.Methods:N-octanol was used as an extractant and ethanol was used as a dispersing agent. The phenylglycolic acid and phenylglyoxylic acid in the urine were extracted, and the upper liquid was taken after vortexing and centrifuged, and then was injected into HPLC for analysis.Results:The linear correlation coefficient of the concentration of phenylglycolic acid in the range of 0~10.0 mg/L was greater than 0.999. The detection limit of the method was 9.9 μg/L, the recovery rates were 86.1%~101.6%. The intraday RSDs of the method were 1.07%~3.76%, and the interday RSDs were 1.24%~3.33%. The linear correlation coefficient of phenylglyoxylic acid in the range of 0.0~2.0 mg/L is greater than 0.999. The detection limit of the method was 2.6 μg/L, the recovery rates were 88.8%~100.3%. The intraday RSDs of the method were 1.02%~ 3.17%, and the interday RSDs were 1.59%~2.41%.Conclusion:The method has low detection limit, high enrichment ratio and good sensitivity, and is suitable for determination of phenylglycolic acid and phenylglyoxylic acid in urine of occupational exposure to styrene.
