Study of methylation of mitochondrial MT-COI of benzene poisoning
10.3760/cma.j.cn121094-20200409-00174
- VernacularTitle:职业性慢性苯中毒患者线粒体细胞色素c氧化酶亚单位I基因甲基化水平的研究
- Author:
Dianpeng WANG
1
;
Diya CAI
;
Xiangli YANG
;
Xin LU
;
Dafeng LIN
;
Peimao LI
;
Zhimin ZHANG
;
Yanfang ZHANG
;
Wen ZHANG
Author Information
1. 518001 深圳市职业病防治院
- Keywords:
Poisoning;
Benzene;
Cytochrome oxidase;
DNA methylation;
Mitochondrial
- From:
Chinese Journal of Industrial Hygiene and Occupational Diseases
2020;38(9):664-668
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To research the mitochondrial cytochrome c oxidase subunit I (MT-COI) gene methylation levels in patients with occupational chronic benzene poisoning, and to explore effective molec μlar biomarkers in patients with occupational chronic benzene poisoning.Methods:38 confirmed cases of occupational chronic benzene poisoning were selected in the case group. 46 healthy people who underwent physical in our hospital were selected in the control group. Pyrosequencing was used to detect the methylation sites of methylation sites, flow cytometry was used to detect peripheral blood cell count levels, and non-parametric statistical methods were used to analyze the differences in detection results between the two groups.Results:The methylation level of mitochondrial MT-COI site 1 (2.21±0.81) % in the case group was less than that in the control group, and the difference was statistically significant ( P<0.05) . The methylation level of mitochondrial MT-COI site 2 (2.31±0.96%) in the case group was less than that in the control group, and the difference was statistically significant ( P<0.05) . The methylation average level of mitochondrial MT-COI (2.26±0.75) % in the case group was less than that in the control group, and the difference was statistically significant ( P<0.05) . Analysis of the average level of methylation found that the methylation level of mitochondrial MT-COI was correlated with WBC ( P<0.05) . Analysis of the average level of methylation found that the methylation level of mitochondrial MT-COI was correlated with platelets ( r=0.254、0.280, P<0.05) . Conclusion:The level of mitochondrial MT-COI gene methylation in patients with occupational chronic benzene poisoning may be related to the sensitivity to benzene exposure. Mitochondrial MT-COI gene methylation may serve as a potential predictive biomarker for benzene poisoning.
