Study on the pharmacokinetics of bromadiolone in oral poisoning rabbits
10.3760/cma.j.cn121094-20190927-00395
- VernacularTitle:家兔经口染毒溴敌隆的代谢动力学研究
- Author:
Shaofeng JIANG
1
;
Yutao ZHANG
;
Jian SUN
;
Jing ZHANG
;
Hongshun ZHANG
Author Information
1. 100050 北京，中国疾病预防控制中心职业卫生与中毒控制所
- Keywords:
Poisoning;
Bromadiolone;
Rabbits;
Pharmacokinetics
- From:
Chinese Journal of Industrial Hygiene and Occupational Diseases
2020;38(6):444-446
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To investigate the variations of bromadiolone concentration in blood and its metabolism in rabbits after oral administration of bromadiolone, and to provide reference for the study of bromadiolone metabolism.Methods:Designed absolute alcohol (1 g/kg) reagent control group, high dose (0.3 mg/kg) and low dose group (0.05 mg/kg) , there were 6 rabbits in each group. Blood samples were collected from the rabbit central auricular artery at regular intervals as 1 h, 2 h, 4 h, 8 h, 12 h, 16 h, 24 h, 36 h, 48 h, 72 h, 168 h, 336 h, 504 h after oral administration. The samples were centrifuged within 1 h. Prothrombin time (PT) , activated partial thromboplastin time (APTT) and concentrations of bromadiolone in plasma were tested. Metabolic kinetics data was analyzed by DAS 3.0.2 software.Results:Bromadiolone had no significant effect on the body weight of the experimental rabbits during the experimental period ( P>0.05) . PT and APTT were significantly abnormal in different dose groups, but for occurrence of exception, PT was earlier than APTT. The concentration of bromadiolone in plasma reached the peak value 12 h after gavage in both high-dose and low-dose groups. The absorption time of t1/2Ka in high-dose group was 4.34 h, the clearance time of t1/2 was 81.52 h, the absorption time of t1/2Ka in low-dose group was 6.90 h, and the elimination time of t1/2 was 56.38 h. The atrioventricular model of bromadidone was three compartment model in rabbits. Conclusion:Bromadiolone can be absorbed rapidly by oral administration, but its metabolism is slow. The change of bromadiolone in vivo accords with the three compartment model.
