Serum metabolomics in latent pneumoconiosis tuberculosis patients based on ultra performance liquid chromatography tandem quadrupole time of flight mass spectrometry
10.3760/cma.j.cn121094-20190613-00239
- VernacularTitle:基于UPLC-QTOF/MS的尘肺潜伏性结核患者血清非靶向代谢组学分析
- Author:
Fangda PENG
1
;
Yijie WANG
;
Bo ZHANG
;
Hongliang GAO
;
Aihua TANG
;
Qingjun QIAN
;
Chunguang DING
Author Information
1. 102308 北京，国家卫生健康委职业安全卫生研究中心（国家卫生健康委煤炭工业职业医学研究中心）
- Keywords:
Pneumoconiosis;
Latent tuberculosis;
Ultra performance liquid chromatography tandem quadrupole time of flight mass spectrometry (UPLC-QTOF/MS);
Metabolom
- From:
Chinese Journal of Industrial Hygiene and Occupational Diseases
2020;38(4):246-250
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To explore the non-target metabonomics of serum in worker's pneumoconiosis (CWP) patients with latent tuberculosis and the biomarkers of latent tuberculosis infection of pneumoconiosis.Methods:In December 2018, 39 CWP inpatients from a hospital in Beijing were taken as subjects. The subjects were screened for latent tuberculosis using the in vitro release test of mycobacterium tuberculosis-interferon (IGRAs) test. According to the screening results, 21 positive patients with latent tuberculosis infection were selected as the latent tuberculosis group of pneumoconiosis. While 18 negative patients with CWP alone were selected as the pneumoconiosis group. Polarity components of metabolites were analyzed by UPLC-QTOF/MS. The data was processed with Progenesis QI software for multidimensional statistical analysis. Identification of structure of differential metabolites were matched through accurate mass and secondary mass spectrum. Searching the Human Metabolome Database (HMDB) , differential metabolites were imported into MetaboAnalyst 4.0 to analyze the metabolic pathways.Results:All 42 differential metabolites were screened out. Excepted for exogenous metabolites, 14 endogenous differential metabolites were identified. Compared with the pneumoconiosis group, 6 metabolites including PC [18∶4 (6Z, 9Z, 12Z, 15Z) /P-18∶1 (11Z) ], 3-Oxododecanoyl-CoA in the latent tuberculosis group were up-regulated, while 8 metabolites including the Stearoyl-CoA, (2S) -Pristanoyl-CoA were down-regulated. These results might be related to lipid, fatty acid and arachidonic acid metabolism pathways.Conclusion:There are significant differences in serum metabonomics between the patients with latent tuberculosis of pneumoconiosis and the patients with ordinary pneumoconiosis, which provide a reference for the study of biomarkers for the diagnosis of latent tuberculosis infection of pneumoconiosis.
