The Function of CD40/CD40L Pathway in Silicosis Fibrosis
10.3760/cma.j.issn.1001-9391.2020.02.004
- VernacularTitle:CD40-CD40L交联系统在矽肺纤维化形成中的作用机制
- Author:
Shujuan WANG
1
;
Kui HU
;
Jingyin HAN
;
Xiaoyu GAN
;
Yi LOU
;
Guohui LI
Author Information
1. 310003 杭州，浙江省中西医结合医院职业病科，浙江
- Keywords:
Silicosis;
Fibrosis;
CD40/CD40L;
Collagen I;
α-SMA
- From:
Chinese Journal of Industrial Hygiene and Occupational Diseases
2020;38(2):96-100
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To investigate the role of CD40/CD40L Pathway in the formation of silicosis fibrosis.Methods:Totally 64 inpatients were recruited and assigned to the silicosis group and the control group, 23 in each group. The alveolar lavage fluid was collected from all patients and isolated. The expression of CD40L protein was detected by Flow Cytometry. The level of IL-8、The IL-6、INF-γ and MCP-1 was detected by ELISA. Two groups of BALF were co-cultured with HFL-1 cells, the expression of Collagen I and α-SMA was detected by Immunohistochemistry.Results:Compared with the control group, CD40L was highly expressed on T lymphocyte cells in silicosis group ( P<0.05) , and the contents of IL-8、The IL-6、INF-γand MCP-1 in Silicosis group were significantly higher than those in control group ( P<0.05) . After co-culture of BALF and HFL-1 cells, the expression levels of Collagen I and α-SMA in Silicosis group were significantly higher than those in control group ( P<0.05) . Conclusion:CD40-CD40L cross-linking system can promote the activation of T cells, release inflammatory factors, promote the synthesis of collagen I and α-SMA by fibroblasts, make the lung fibrous tissue proliferate, and lead to the formation of silicosis fibrosis.
