Protective effect of Nrf2 activation by curcumin against lead-induced toxicity and apoptosis in SH-SY5Y cells
10.3760/cma.j.issn.1001-9391.2016.06.001
- VernacularTitle:姜黄素激活Nrf2对铅致SH-SY5Y细胞毒性和凋亡的保护作用研究
- Author:
Fen LI
1
;
Zhen SONG
;
Lichao BO
;
Ling LV
;
Jun CHEN
Author Information
1. 300383,天津市西青区疾病预防控制中心
- Keywords:
Lead acetate;
SH-SY5Y cell;
Apoptosis;
Curcumin;
Nuclear factor erythroid 2-related factor 2
- From:
Chinese Journal of Industrial Hygiene and Occupational Diseases
2016;34(6):401-405
- CountryChina
- Language:Chinese
-
Abstract:
Objective To study the protective effect of nuclear factor erythroid 2-related factor 2 (Nrf2)activation by curcumin against lead-induced toxicity and apoptosis in SH-SY5Y human neuroblastoma cells and its impact on expression of apoptosis-related proteins.Methods After the cells were treated with 0,0.5,1,5,or 10 μmol/L curcumin for 24 hours,nucleoprotein was extracted and electrophoretic mobility shift assay was used to measure Nrf2-antioxidant responsive element (ARE) binding capacity.The optimal concentration of curcumin was figured out for treating cells.After pretreatment with 5 μmol/L curcumin for 24 hours,cells were exposed to lead acetate at different concentrations (0,5,25,and 125 μmol/L for control,low-dose,mediumdose,and high-dose groups).The 3-[4,5-dimethylthiazol-2-yl]-2,5-diphenyl tetrazolium bromide (MTT) assay,flow cytometry,and Western blot were used to evaluate cell viability,apoptosis,and expression of apoptosisrelated proteins,respectively.Results Curcumin of 5 μmol/L caused significantly increased cell viability in low-,medium-,and high-dose groups exposed to lead acetate for 12 hours(98.42％±1.12％ vs 92.92％±0.14％,P＜0.05;95.30％±1.17％ vs 91.15％±0.67％,P＜0.05;94.50％±1.45％ vs 85.98％±0.45％,P＜0.05).Curcumin of 5 μmol/L also caused significantly increased cell viability in medium-and high-dose groups exposed to lead acetate for 24 hours (93.10％±1.63％ vs 88.40％±4.13％,P＜0.05;90.13％±2.03％ vs 83.63％±3.42％,P＜0.05).The high-dose group had a significantly higher apoptotic rate than the control group 6.17％ ±1.31％ vs 3.30％±0.53％,P＜0.05).Curcumin of 5.0 μmol/L significantly reduced the apoptotic rate in the high-dose group (2.97％±0.15％ vs 6.17％±1.31％,P＜0.05).Exposure to lead acetate elevated the expression of Bax,cytochrome C,and caspase-3 and reduced Bcl-2 expression.Curcumin of 5.0 μmol/L significantly reduced the expression of Bax,cytochrome C,and caspase-3 in the high-dose group (P＜0.05).Conclusion Nrf2 activation by curcumin has a protective effect against lead-induced toxicity and apoptosis in SH-SY5Y cells.The protective effect of Nrf2 against apoptosis may be associated with the regulation of apoptosis-related proteins.
