The effect of paraquat on autophagy in human embryonic neural progenitor cells
10.3760/cma.j.issn.1001-9391.2016.03.005
- VernacularTitle:百草枯对人胚胎神经祖细胞自噬作用的影响
- Author:
Lina ZHAO
1
;
Mengling YAN
;
Xinjin WANG
;
Tinging DOU
;
Xiuli CHANG
;
Zhijun ZHOU
Author Information
1. 复旦大学公共卫生学院/教育部公共卫生重点实验室/WHO职业卫生合作中心/健康风险预警治理协同创新中心
- Keywords:
Paraquat;
Neurous;
Stem cell;
Autophagy
- From:
Chinese Journal of Industrial Hygiene and Occupational Diseases
2016;34(3):178-183
- CountryChina
- Language:Chinese
-
Abstract:
Objective To explore the effect of paraquat (PQ) on autophagy in human embryonic neural progenitor cells.Methods Using ReNcell CX cell model.After treatment with various concentration (0.00,1.00,10.00 and 100.00 μmol/L) of PQ,CCK8 assay was used to detect the cell viability,the transmission electron microscope was used to observe the the cell ultrastructure,the real-time polymerase chain reaction (RTPCR) was adopted to detect mRNA expression of autophagy related genes which including LC3,Atg12,Atg5,beclinl,Atg7 and mTOR and apoptosis related genes Bax and Bcl-2.Results The cell viability was significantly inhibited after administered with 100.00 μmol/L of PQ (P＜0.01).The mRNA expression of Beclin1 was significantly up-regulated and the emergency of autophagosome were observed at the concentration of 1.00 μ mol/L group,while mild cell apoptosis,significantly up-regulated Atg5,Atg8,Atg7 and Atg12 mRNA expression as well as down-regulated expression of mTOR and Bax were detected at the 10.00 μmol/L of PQ group,howere,the obvious apoptosis and the up-regulated mRNA expression of mTOR and Bax were observed at the 100.00 μmol/L of PQ group,the mRNA expression of Bcl-2 were all down-regulated after administered with 1.00,10.00 and 100.00 μmol/L of PQ and reached the lowest level at the concentration of 10.00 μmol/L.Conclusion PQ can induced autophagy at the low concentration in ReNcell CX cell and autophagy might serve as a protective mechanism to ameliorate PQ-induced cytotoxic effects but apoptosis will be induced at the 100 μmol/L concentration.
