Nickel smelting dust exposures to NIH/3T3 cellular mitochondrial damage and L-ascorbic acid interference effect
10.3760/cma.j.issn.1001-9391.2016.03.003
- VernacularTitle:镍冶炼烟尘染毒致NIH/3T3细胞线粒体损伤及L-抗坏血酸的干预作用
- Author:
Yulin PAN
1
;
Yue WANG
;
Yao FU
;
Shengyuan WANG
;
Wenqiao TAN
;
Jiashu CHEN
;
Yonghui WU
Author Information
1. 150081,哈尔滨医科大学公共卫生学院劳动卫生与职业病学教研室
- Keywords:
Nickel;
Smelting dust;
Mitochondrial;
L-ascorbic acid
- From:
Chinese Journal of Industrial Hygiene and Occupational Diseases
2016;34(3):173-177
- CountryChina
- Language:Chinese
-
Abstract:
Objective To study the protection of L-ascorbic acid on mouse embryonic fibroblasts(NIH/ 3T3) from carcinogenic effects caused by nickel smelting smoke subjects.Methods The NIH/3T3 cells were divided into experimental and L-ascorbic acid in the intervention group.Plus exposure group concentration of nickel refining dusts were formulate 0.00,25.00,50.00,100.00 μg/ml suspension,the intervention group on the basis of the added exposure group containing L-ascorbic acid (100 mmol/L),contacted.Then,the cell viability was detected by MTT assay,we used Calcein-AM fluorescence probe to detect cell mitochondrial permeability transition pore (MPTP) changes,JC-1 staining to observe and detect the cell mitochondrial membrane potential (MMP) change,colorimetric quantitative to study the activity of mitochondrial respiratory chain complex Ⅰ,l,Ⅲ,Ⅳ.Results Upon 24 h incubation,both cell relative inhibition rate,openness of MPTP were increasing enhanced by different concentrations,on the other hand,MMP and the activity of mitochondrial respiratory chain complex Ⅰ,Ⅱ,Ⅳ were obviously decreased,the differences were statistically significant (P＜0.05).After L-ascorbic acid intervention treatment,the results of the intervention group were lower than that of the exposure group,and the difference was statistically significant (P＜0.05),indicating the protection of L-ascorbic acid on cell mitochondrial from the nickel exposure damage.Conclusion The damage effects of nickel on NIH/3T3 cell mitochondrial was significantly enhanced with the increasing concentration,and L-ascorbic acid has certain protection on cellular mitochondrial.
