Gas chromatography for measurement of chlorpyrifos in serum
10.3760/cma.j.issn.1001-9391.2016.02.016
- VernacularTitle:血清中毒死蜱测定的气相色谱法
- Author:
Jingjin ZHAO
1
;
Yi QIAN
;
Shuming DU
Author Information
1. 300171,天津市职业病防治院
- Keywords:
Chlorpyrifos;
Poisoning;
Serum;
Chromatography,gas
- From:
Chinese Journal of Industrial Hygiene and Occupational Diseases
2016;34(2):138-139
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the method for the measurement of chlorpyrifos in serum by gas chromatography,and to provide a basis for emergency treatment of poisoning in clinical practice.Methods Venous blood (3.0 ml) was collected from patients.After coagulation,the blood samples were centrifuged at 4 000 r/min for 5 minutes,and 0.5 ml of serum was placed in a glass test tube with a cork;4.0 ml of ethyl acetate was then added and mixed rapidly,and this solution was subjected to extraction for 5 minutes and centrifuged at 4 000 r/min for 10 minutes.The ethyl acetate layer was placed in a conical tube and extracted twice with the same method.The extract was mixed and blow-dried with nitrogen,and the residue was dissolved with 50.0 μl ethanol.Gas chromatography was used for measurement,with a sample size of 1 μl and a retention time of 9.609 minutes.Results The linear range of this method was 0.2～20.0 μg/ml,and the regression equation was y =2 372.6x+357.2 (r=0.999 6).The detection limit of chlorpyrifos in serum was 0.05 μg/ml,and the recovery rate was 84.6％～102.4％.The relative standard deviation was 3.6％～4.8％,and the intra-day and inter-day relative standard deviations were 3.62％～5.10％ and 3.77％～4.98％,respectively.Conclusion This detection method is accurate,simple,and convenient,and can be used for the clinical diagnosis of chlorpyrifos poisoning.
