Observation on the injury of tissues and cells in rats with acute nickel carbonyl poisoning
10.3760/cma.j.issn.1001-9391.2011.09.020
- VernacularTitle:急性羰基镍中毒大鼠肺组织和细胞损伤的实验观察
- Author:
Ning CHENG
1
;
Qiu-Ying WANG
;
Yu LI
;
Bei WANG
;
Xiao-Qiang XUAN
;
Jing LIU
;
Xi-Jiang WU
Author Information
1. 兰州大学
- Keywords:
Nickel carbonyl;
Lung;
DNA damage
- From:
Chinese Journal of Industrial Hygiene and Occupational Diseases
2011;29(9):705-706
- CountryChina
- Language:Chinese
-
Abstract:
Objective To observe DNA damage and morphological changes of lung cells in rats with acute carbonyl nickel poisoning. Methods SD rats were exposed to 20, 135 and 250 mg/m3 carbonyl nickel for 30 min by inhalation. On the third day and 7th day after exposure, DNA damage of lung cells were examined by single cell gel electrophoresis (SCGE) and the pathological changes in lung tissues and the changes of microstructure in lung cells were observed. Results The DNA damage of the rat lung cells were obviously found in all exposure groups at different exposure times and there was the most obvious DNA damage at 72 hour after exposure to nickel carbonyl but the damage appeared later than chlorine exposure group. The inflammatory infiltration and hyperplasia in lung tissue, bronchiolar damage and the bronchial mucosa defulvium appeared in the rats exposed to carbonyl nickel. The results of microstructure examination indicated that the organelles of type I pneumocytes was swellen, the body of type Ⅱ pneumocytes was emptied. The cytoplasm empty bubbles increased, the mitochondria was swellen, and alveolar mediastinum inside the collagen fiber in alveolar mediastinum increased. Conclusion The acute carbonyl nickel exposure could induce the injury of lung tissues and cells with dose-effect relationship and time-effect relationship.
