The influence of carbon disulfide on hydroxyl free radicals in phenanthroline chemiluminescence system
10.3760/cma.j.issn.1001-9391.2001.02.010
- VernacularTitle:二硫化碳对邻菲罗啉化学发光体系中羟自由基产生的影响
- Author:
LE JIAN
1
Author Information
1. 浙江大学
- From:
Chinese Journal of Industrial Hygiene and Occupational Diseases
2001;19(2):112-114
- CountryChina
- Language:Chinese
-
Abstract:
Objective To clarify the effects of carbon disulfide(CS2) on hydroxyl free radical(*OH) generation in phenanthroline chemiluminescence system(Phen-system). Methods The dynamic curve of various concentrations of CS2,the quenching effect of thiourea on chemiluminescence intensity and the influence of CS2 on Phen-system without CuSO4 and Vit C were observed with phenanthroline chemiluminescence system. Results CS2 could enhance the emission intensity of chemiluminescence and advance the peak time.It had dose-effect relationship between CS2 concentrations (0,10,40,80,120,160 mg/ml) and chemiluminescence peak (r=0.955,R2=0.913，P=0.003),as well as CS2 concentrations and peak time(r=-0.927，R2=0.860，P=0.008).The enhanced chemiluminescence induced by CS2 could be quenched by thiourea but the inhibition rate of it reduced along with the increasing of CS2 concentration(r=-0.816，R2=0.666，P=0.048).When the system were free of CuSO4 and Vit C,CS2 could also induce the emission of luminous intensity. Conclusion CS2 could induce the generation of hydroxyl free radicals and advance the peak time in Phen-system.
