The effect of dimethylformamide on 45 Ca transport in rat kidney mitochondria and microsomes in vitro
10.3760/cma.j.issn.1001-9391.2001.01.021
- VernacularTitle:二甲基甲酰胺对大鼠肾脏线粒体和微粒体45 Ca转运的影响
- Author:
Xiangdong JIAN
1
;
Baotian KAN
;
Xiulian ZHANG
Author Information
1. The Affiliated Hospital to Shandong Med ical University
- From:
Chinese Journal of Industrial Hygiene and Occupational Diseases
2001;19(1):62-64
- CountryChina
- Language:Chinese
-
Abstract:
Objective To observe the influence of dimet hylformamide(DMF) on 45 Ca transport in rat kidney mictochondria an d microsomes. Methods Mitochondria and microsomes were is olated by ultracentrifugation.Radioactive isotope technique was used to examine the effects of DMF on 45 Ca uptake and release of mitochondria and microsomes and to explore the mechanism of renal injury. Results (1)The 45 Ca uptake of mitochondria in DMF groups(130 μmol/L ,195 μmol/L) after 10 min incubation were (4.20±0.29)nmol/mg pro and (3.81± 0.42)nmol/mg pro respectively(P<0.05 or P<0.01),compared with control group (5.18±0.40)nmol/mg pro,and the 45 Ca uptake of microsomes in D MF groups(130 μmol/L,195 μmol/L) after 10 min incubation were (2.10±0.42) nmol/mg pro,(2.01±0.38)nmol/mg pro respectively(P<0.05 or P<0.01),compa red with control group (2.96±0.38)nmol/mg pro.The same results were gained afte r 20 min and 30 min incubation.(2)The 45 Ca remain in mitochondria of DMF groups(65 μmol/L,130 μmol/L,195 μmol/L) after 2 min incubation were (5.4 0±0.39)nmol/mg pro,(5.03±0.32)nmol/mg pro and (4.70±0.50)nmol/mg pro resp ectively(P<0.01),compared with control group［(6.01±0.40)nmol/mg pro］. The 45 Ca remain in microsomes of DMF groups(65 μmol/L,130 μmol/L,1 95 μmol/L) after 2 min incubation were (3.43±0.26)nmol/mg pro,(3.18±0.31) nmol/mg pro and (3.06±0.27)nmol/mg pro respectively(P<0.01),compare d with control group (4.06±0.32)nmol/mg pro,indicating increased release of calcium.The same results were gained 4 min and 8 min after incubation.There wer e dose-effect relationships between DMF and 45 Ca uptake or 45 Ca release in both mitochondria and microsomes(r=0.918,0.895,0.886,0.8 46 respectively). Conclusion DMF may induce disturbance of calcium homeost asis in rat kidney.
