High expression of miR-204-5p promotes malignant behaviors of bladder cancer cells by negatively regulating RAB22A
10.12122/j.issn.1673-4254.2024.11.21
- VernacularTitle:miR-204-5p通过靶向负性调控RAB22A促进膀胱癌细胞的恶性生物学行为
- Author:
Liqiang LI
1
;
Yuanyuan GUO
;
Chengyong WANG
;
Rui CHANG
;
Wei SUN
;
Wuyue GAO
;
Chao WANG
;
Beibei LIU
Author Information
1. 蚌埠医科大学第一附属医院泌尿外科,安徽 蚌埠 233004
- Keywords:
miR-204-5p;
RAB22A;
bladder cancer
- From:
Journal of Southern Medical University
2024;44(11):2235-2242
- CountryChina
- Language:Chinese
-
Abstract:
Objective To explore the regulatory effect of miR-204-5p on biological behaviors of bladder cancer cells and its molecular mechanism.Methods Survival analysis and correlation analysis were performed using TCGA database to explore the association of miR-204-5p expression with survival outcomes and clinicopathological parameters of bladder cancer patients.The expression level of miR-204-5p was detected in bladder cancer and adjacent tissues and in normal uroepithelial cells and bladder cancer cells.In cultured bladder cancer cells,the effects of miR-204-5p overexpression and knockdown on cell proliferation,migration,invasion,and apoptosis were analyzed.Transcriptome sequencing,bioinformatics analysis and dual-luciferase assay were carried out to confirm targeted inhibition of RAB22A by miR-204-5p to promote malignant biological behaviors of bladder cancer cells.Results Patients with high miR-204-5p expressions had lowered median survival time and poor prognosis(P<0.05).The expression of miR-204-5p was significantly up-regulated in bladder cancer tissues and cells(P<0.05).In bladder cancer cells,miR-204-5p overexpression significantly promoted cell proliferation,migration and invasion and reduced cell apoptosis.Transcriptome sequencing,bioinformatics analysis and dual-luciferase assay all suggested that RAB22A was a key downstream factor of miR-204-5p.Overexpression of miR-204-5p significantly inhibited RAB22A expression in bladder cancer cells,and overexpression of RAB22A partially reversed miR-204-5p overexpression-induced enhancement of bladder cancer cell proliferation.Conclusion High expression of miR-204-5p promotes proliferation,migration and invasion and reduces apoptosis of bladder cancer cells by negatively regulating RAB22A expression.
