Quercetin ameliorates diabetic kidney injury in rats by inhibiting the HMGB1/RAGE/NF-κB signaling pathway
10.12122/j.issn.1673-4254.2024.09.17
- VernacularTitle:槲皮素通过抑制HMGB1/RAGE/NF-κB信号通路减轻糖尿病引起的大鼠肾脏损伤
- Author:
Yifan JIANG
1
;
Xiaorong LI
;
Jiayi GENG
;
Yongfeng CHEN
;
Bi TANG
;
Pinfang KANG
Author Information
1. 蚌埠医科大学临床医学院,安徽 蚌埠 233000
- Keywords:
quercetin;
diabetic nephropathy;
HMGB1/RAGE/NF-κB signaling pathway
- From:
Journal of Southern Medical University
2024;44(9):1769-1775
- CountryChina
- Language:Chinese
-
Abstract:
Objective To explore the effect of quercetin on renal inflammation and cell apoptosis in diabetic rats and its possible mechanisms.Methods Twenty-four adult male SD rats were randomized equally into normal control group,high-glucose and high-fat feeding group,streptozotocin(STZ)-induced diabetic model group,and quercetin treatment(daily dose 100 mg/kg)group.Pathological changes of the renal tissues of the rats were observed with HE staining,serum inflammatory factor levels were determined with ELISA,and renal expression of NF-κB was observed by immunohistochemistry.Fast blood glucose(FBG),serum levels of triglyceride(TG),BUN,and Scr,and 24-h urine protein content of the rats were measured,and renal expressions of HMGB1,RAGE,NF-κB,Bax,Bcl-2,and caspase-3 were detected with Western blotting.Results The diabetic rats showed significantly increased levels of FBG,TG,BUN,and Scr,renal hypertrophy index,24-h urinary protein content,serum IL-1β,IL-6 and TNF-α levels and renal expressions HMGB1,RAGE,NF-κB,Bax,and caspase-3 with decreased renal expression of Bcl-2.All these changes were significantly alleviated by quercetin treatment of the rats.Conclusion Quercetin can ameliorate kidney injury in diabetic rats possibly by inhibiting the HMGB1/RAGE/NF-κB inflammatory signaling pathway to reduce renal inflammation and renal cell apoptosis.
