Yigong San improves cognitive decline in a rat model of Alzheimer's disease by regulating intestinal microorganisms
10.12122/j.issn.1673-4254.2024.07.09
- VernacularTitle:异功散通过调控肠道微生物改善痴呆症模型大鼠的认知下降
- Author:
Jing ZENG
1
,
2
;
Rong CHEN
;
Xiangyi REN
;
Lei HUA
;
Yong YANG
;
Jiangping WEI
;
Xiaomei ZHANG
Author Information
1. 中药新药创制川渝共建重点实验室//重庆市中药研究院,重庆 400065
2. 国家中医药管理局中药化学三级实验室//重庆市中药研究院,重庆 400065
- Keywords:
Yigong San;
lipopolysaccharide;
cognitive decline;
intestinal microbiota
- From:
Journal of Southern Medical University
2024;44(7):1297-1305
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the effect of Yigong San(YGS)on learning and memory abilities of rats with lipopolysaccharide(LPS)-induced cognitive decline and explore its possible mechanism in light of intestinal microbiota.Methods Forty SD rats were randomly divided into control group,model group,donepezil(1.3 mg/kg)group,and high-dose(5.25 g/kg)and low-dose(2.63 g/kg)YGS treatment groups.After 24 days of treatment with the corresponding drugs or water by gavage,the rats in the latter 4 groups received an intraperitoneal injection of LPS(0.5 mg/kg)to establish models of Alzheimer's disease(AD).Water maze test and HE staining were used to evaluate the changes in learning and memory abilities and pathomorphology of the hippocampus.The changes in gut microbial species of the rats were analyzed with 16S rRNA sequencing,and the levels of IL-6,TNF-α,and IL-1β in the brain tissue and serum were detected using ELISA.Results Compared with the AD model group,the YGS-treated rats showed significantly shortened escape latency on day 5 after modeling,reduced neuronal degeneration and necrosis in the hippocampus,lowered pathological score of cell damage,and decreased levels IL-6,TNF-α and IL-1β in the brain tissue and serum.The YGS-treated rats showed also obvious reduction of Alpha diversity indicators(ACE and Chao1)of intestinal microbiota with significantly increased abundance of Prevotellaceae species at the family level and decreased abundance of Desulfovibrionaceae,which were involved in such metabolic signaling pathways as cell community prokaryotes,membrane transport,and energy metabolism.Conclusion YGS improves learning and memory abilities and hippocampal pathomorphology in AD rat models possibly by regulating the abundance of intestinal microbial species such as Prevotellaceae to affect the metabolic pathways for signal transduction,cofactors,and vitamin metabolism.
