MiR-26-3p regulates proliferation,migration,invasion and apoptosis of glioma cells by targeting CREB1
10.12122/j.issn.1673-4254.2024.03.20
- VernacularTitle:miR-26b-3p靶向CREB1调控神经胶质瘤细胞的增殖、迁移及侵袭
- Author:
Qiuhu HUANG
1
;
Jian ZHOU
;
Zizhen WANG
;
Kun YANG
;
Zhenggang CHEN
Author Information
1. 海南医学院第一附属医院神经外科,海南 海口 570102
- Keywords:
cAMP-responsive element binding protein 1;
miR-26b-3p;
glioma;
proliferation;
invasion;
migration
- From:
Journal of Southern Medical University
2024;44(3):578-584
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the regulatory role of miR-26b-3p in proliferation,migration and invasion of glioma.Methods The expressions of miR-26b-3p and cAMP-responsive element binding protein 1(CREB1)in gliomas of different pathological grades were detected with RT-qPCR and Western blotting.Bioinformatic methods were used to analyze the target sequence of miRNA-26b-3p binding to CREB1,and dual luciferase gene reporter experiment was performed to explore the mechanism for targeted regulation of CREB1 by miR-26b-3p.Glioma U251 cells were treated with miR-26b-3p mimic or inhibitor,and the changes in CREB1 expression and cell proliferation,migration,invasion and apoptosis were determined with Western blotting,CCK-8 assay,wound healing assay,Transwell assay,and flow cytometry.Results The expression of miR-26b-3p decreased while CREB1 expression increased significantly as the pathological grade of gliomas increased(P<0.05).Dual luciferase gene reporter experiment confirmed that CREB1 was a downstream target of miR-26b-3p.Inhibition of miR-26b-3p significantly upregulated the expression of CERB1,suppressed apoptosis and promoted proliferation and invasion of glioma cells,and overexpression of miR-26b-3p produced the opposite effects(P<0.05).Conclusion MiR-26b-3p regulates CREB1 expression to modulate apoptosis,proliferation,migration and invasion of glioma cells,thereby participating in tumorigenesis and progression of glioma.
