Chlorogenic acid alleviates acute kidney injury in septic mice by inhibiting NLRP3 inflammasomes and the caspase-1 canonical pyroptosis pathway
10.12122/j.issn.1673-4254.2024.02.14
- VernacularTitle:绿原酸减轻脓毒症诱导的小鼠急性肾损伤:基于抑制caspase-1经典细胞焦亡信号通路
- Author:
Shangping FANG
1
,
2
;
Renke SUN
;
Hui SU
;
Kecheng ZHAI
;
Yu XIANG
;
Yangmengna GAO
;
Wenjun GUO
Author Information
1. 皖南医学院麻醉学院,安徽 芜湖 241002
2. 皖南医学院麻醉学实验实训中心,安徽 芜湖 241002
- Keywords:
chlorogenic acid;
acute kidney injury;
sepsis;
cell pyroptosis;
NLRP3 inflammasomes
- From:
Journal of Southern Medical University
2024;44(2):317-323
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the role of caspase-1-medicated canonical pyroptosis pathway in chlorogenic acid(CGA)treatment of acute kidney injury(AKI)in mice.Method Twenty-four C57Bl/6J mice were randomized into sham-operated group,cecal ligation and puncture(CLP)group,CLP+dexamethasone group(CLP+DXM group),and CLP+CGA group(n=6)and subjected to either sham operation(laparotomy only)or CLP.After modeling the mice received intravenous infusion of 10 mg/kg normal saline(in sham and CLP groups),1 μg/kg dexamethasone or 15 mg/kg of chlorogenic acid for 6 h delivered using an intravenous pump.Eight hours after the infusion,renal morphology and histology,renal cell apoptosis,and the renal function parameters such as urea nitrogen(BUN),creatinine(Scr),and kidney injury molecule 1(KIM-1)were compared among the 4 groups;the 7-day survival rates of the mice were recorded,and the expressions of NLRP3 inflammasomes and key proteins of the caspase-1 pathway in the renal tissue were detected.Results CGA treatment significantly improved the 7-day survival rate,reduced renal pathologies of the septic mice(P<0.05),and lowered the levels of BUN,Scr,KIM-1,NLRP3 inflammasome and expressions of key proteins of the caspase-1 pathway.Conclusion CGA alleviates AKI in mice with CLP-induced sepsis by inhibiting NLRP3 inflammasomes and the caspase-1 signaling pathway.
