Efficacy of navel application of Jianpiwenyang Gel for chronic diarrhea of spleen and stomach weakness type:a randomized controlled trial and analysis of the mechanism
10.12122/j.issn.1673-4254.2024.02.03
- VernacularTitle:健脾温阳凝胶剂脐疗治疗脾胃虚弱型慢性腹泻的疗效及机制:一项临床随机对照试验
- Author:
Yixin CUI
1
;
Decai WANG
;
Dongqing XIE
;
Haiming WANG
;
Ruixin XU
;
Xiaoran TANG
;
Yin ZHANG
Author Information
1. 中国人民解放军总医院 第六医学中心中医医学部,北京 100853
- Keywords:
Jianpiwenyang Gel;
chronic diarrhea;
navel therapy;
network pharmacology;
molecular docking
- From:
Journal of Southern Medical University
2024;44(2):217-225
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the efficacy of Jianpiwenyang Gel(SSWYG)for treating chronic diarrhea and explore its therapeutic mechanism.Methods Eighty patients with chronic diarrhea of spleen and stomach weakness type were randomized into two groups for interventions with lifestyle adjustment and treatment with bifid triple viable capsules(control group,n=40)or naval application with SSWYG(treatment group,n=40)for one week,after which symptoms of chronic diarrhea were evaluated.The Chinese medicine system pharmacology analysis platform(TCMSP),GeneCards,NCBI,OMIM database and GEO database(GSE14841)were used to obtain the active ingredients and target proteins of SSWYG and chronic diarrhea-related targets.The key targets were obtained by topological analysis for Gene Ontology(GO)and KEGG analyses.The affinity and binding characteristics of SSWYG for specific targets were verified by molecular docking using AutoDock software.Results In both groups,gastrointestinal symptom rating scale(GSRS),Bristol Scale and TCM syndrome scores significantly improved after the treatments(P<0.05),and better effects were observed in the treatment group(P<0.05).Sixty-eight targets of SSWYG in treating chronic diarrhea were obtained,and 33 most probable ones were screened out by topological analysis.GO and KEGG analyses identified several chronic diarrhea-related pathways including the TNF and IL-17 pathways.Molecular docking study showed good affinity of the core components of SSWYG for the key targets CASP3,JNK,IL1B,IL6,and AKT1.JUN and CASP3 had the lowest binding energy and the highest stable binding energy with multiple major active ingredients of SSWYG.Conclusion SSWYG can significantly improve clinical symptoms of chronic diarrhea possibly by regulating the TNF and IL-17 as well as other pathways via CASP3 and JUN,suggesting a complex therapeutic mechanism of SSWYG involving multiple ingredients and targets and coordinated regulation of multiple pathways.
