Osteogenic effect of collagen/bioglass composites carrying noggin siRNA
10.3969/j.issn.1673-4254.2018.01.017
- VernacularTitle:负载siRNA胶原/生物活性玻璃复合材料的成骨作用
- Author:
Yanling CHEN
1
;
Liangjiao CHEN
;
Zhengmao LI
;
Zedong LAN
Author Information
1. 福建医科大学附属口腔医院正畸科
- Keywords:
osteogenesis;
siRNA;
collagen;
bioglass
- From:
Journal of Southern Medical University
2018;38(1):106-111
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate osteogenic effect of collagen/bioglass composites loaded with a small interfering RNA (siRNA) targeting noggin. Methods The collagen/bioglass composites loaded with the negative control siRNA or noggin siRNA were prepared by freeze-drying method. CCK8 test was used to evaluate the proliferation of MC3T3 cells exposed to the aqueous extracts of collagen/bioglass composites and the siRNA-loaded collagen/bioglass composites. ALP activity assay, quantitative real-time PCR and Alizarin Red staining were used to assess the effect of the 3 composites on mineralization in MC3T3 cells. Results MC3T3 cells cultured for 3 and 5 days in the presence of the extracts of the 3 composites all showed significantly more active proliferation than the blank control cells (P<0.05). Compared with the cells seeded on the scaffold without siRNA, MC3T3 cells seeded on collagen/bioglass scaffold loaded with noggin siRNA showed a significantly higher ALP activity at 14 days after seeding (P<0.05) with significantly increased expression of ALP, Runx2 and BSP mRNAs (P<0.05). Alizarin Red staining showed that the cells seeded on the noggin siRNA-loading collagen/bioglass scaffold contained significantly more mineralized nodules than the other cells (P<0.05). Conclusion The collagen/bioglass composites loaded with noggin siRNA have a good biocompatibility, and the collagen/bioglass composites and noggin siRNA show a synergistic effect in promoting osteogenesis.
