Effect of semen bacterial infection on semen parameters and analysis of drug resistance in 74376 male infertility patients
10.3969/j.issn.1673-4254.2018.01.014
- VernacularTitle:精液细菌感染对精液参数及细菌耐药性的影响—附74376例男性不育症患者分析
- Author:
Silu YIN
1
;
Jian HU
;
Weina LI
;
Lin SHI
;
Gang LIU
Author Information
1. 中南大学基础医学院生殖与干细胞工程研究所
- Keywords:
semen quality;
bacterial infection;
drug sensitivity analysis
- From:
Journal of Southern Medical University
2018;38(1):89-94
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the effect of semen bacterial infection on semen parameters in male infertility patients and analyze of the drug resistance profile of the bacterial isolates. Methods According to the results of bacterial culture, the semen samples of 74376 infertile men collected between April, 2016 and April, 2017 were divided into infection group and non-infection group, and the infertility type and semen parameters were compared between the two groups. The bacterial species and the drug sensitivity of the isolates were analyzed. Results Bacterial infections were detected in 1.38％of the total semen samples collected. The positivity rate of semen bacterial infection was 1.41％in normal semen group, 1.55％in asthenospermia group, 1.18％ in oligospermia group, 1.57％ in asthenospermia/ oligospermia group, and 0.17％ in azoospermia group. The positivity rate was lower in azoospermia group than in the other groups. Bacterial infection mainly affected sperm motility (P<0.05), sperm density and forward motile sperm ratio (P<0.01). The most common bacterial species causing the infections included, in the descending order of frequencies, Escherichia coli (63.59％), Klebsiella pneumoniae subspecies (19.80％) and Proteus mirabilis (13.22％). Drug susceptibility tests showed that the isolates of Escherichiacoli, Pneumonia Klebsiella pneumonia subspecies and Citrobacter koseri were commonly resistant to amoxicillin; Pseudomonas aeruginosa isolates were resistant to Bactrim and ampicillin/sulbactam; Staphylococcus aureus isolates were resistant to penicillin. Conclusion Male infertile patients have a low bacterial infection rate in the semen. Bacterial infection severely affects the sperm fertilization process by causing impairment of sperm motility and lowered sperm density. Escherichia coli is the most common pathogenic bacterium for the semen infection. With the exception of Staphylococcus aureus, the other bacterial strains isolated were found to be sensitive to imipenem and meropenem.
