Intra-articular injection of ascorbic acid/ferric chloride relieves cartilage degradation in rats with osteoarthritis
10.3969/j.issn.1673-4254.2018.01.010
- VernacularTitle:关节腔注射抗坏血酸/氯化铁可延缓骨性关节炎大鼠软骨退变
- Author:
Zhenting LIAO
1
;
Zhenquan XING
;
Yufan CHEN
;
Zhonghao DENG
;
Desheng WU
;
Liang ZHAO
Author Information
1. 南方医科大学南方医院关节与骨病外科
- Keywords:
osteoarthritis;
ascorbic acid;
ferric chloride;
intra-articular injection;
fenton reaction;
latent TGFβ1
- From:
Journal of Southern Medical University
2018;38(1):62-68
- CountryChina
- Language:Chinese
-
Abstract:
Objective To assess the effect of ascorbic acid/ferric chloride (AA/FeCl3) in attenuating cartilage damage in rats with osteoarthritis. Methods Thirty adult male Wistar rats with surgically induced osteoarthritis were randomized into 2 groups for treatment with intra-articular injection of saline (control group) or AA/FeCl3 mixture (AA group) once a week starting from the third week after the operation. At 6, 9, and 12 weeks after the operation, 5 rats from each group were sacrificed for observing subchondral bone changes on X-ray films and evaluation of cartilage degeneration in the right knee joints using safranin-O/Fast green staining and a modified OARSI scoring system. The degradation of the cartilage matrix was observed by immunohistochemical staining for type II collagen. Results X-ray examination in saline control group revealed the presence of osteophytes and narrowing of the joint space at 9 weeks, and the joint line disappeared at 12 weeks after the surgery; only slight irregularity of the articular surface was observed in the AA group at 9 and 12 weeks. OARSI scores were significantly lower in AA group than in the control group at 9 weeks (18.67 ± 0.67 vs 12.17 ± 2.75;P<0.05) and 12 weeks (20.11 ± 1.84 vs 13.77 ± 0.40;P<0.05) but not at 6 weeks after the surgery. The content of type 2 collagen in AA group was significantly higher than that in the control group at 6 weeks (0.36 ± 0.039 vs 0.49 ± 0.029;P<0.05) and 9 weeks after the surgery (0.25 ± 0.041 vs 0.38 ± 0.040;P<0.05). Conclusions Early intra-articular injection of AA/FeCl3 can effectively delay the progression of post-traumatic osteoarthritis in rats.
