Effect of HBP-A on meniscal injury and pathological hypertrophy and calcification of the meniscus
10.3969/j.issn.1673-4254.2017.04.02
- VernacularTitle:河蚌葡聚糖对半月板组织损伤及病理性过度肥大和钙化的影响
- Author:
Guoqing DU
1
;
Daofang DING
;
Yuanyuan FENG
;
Linghui LI
;
Tengfei LEI
;
Bo CHEN
;
Zhen DENG
;
Hongsheng ZHAN
Author Information
1. 上海中医药大学附属曙光医院石氏伤科医学中心
- Keywords:
HBP-A;
abnormal loading;
meniscus;
hypertrophy;
calcification
- From:
Journal of Southern Medical University
2017;37(4):431-437
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the effect of HBP-A on meniscal injuries and the expressions of genes associated with pathological hypertrophy and calcification of the meniscusinduced by abnormal loading. Methods Bovine meniscus explants were subjected to 25%strain at 0.3 Hz for 3 h and treated with 0.6 mg/mL of HBP-A. The cell viability in the meniscus explants after 72 hin culture was determined using live/dead staining and the expression levels of genes associated with pathological hypertrophy and calcification of the meniscus (ANKH, ENPP1, ALP, MMP13, and IL-1) were measured using real-time PCR and Western blotting. The conditioned medium was collected for testing sulfated glycosaminoglycan (GAG) release. Results The number of dead cells, loss of proteoglycan content, and the expressions of ANKH, ENPP1, ALP and MMP13, and IL-1 at both the mRNA and protein levels were all significantly lower in the meniscus explants treated with 0.6 mg/mL HBP-A than in the explants with only 25%abnormal pressure stimulation (n=3, P<0.05). Conclusion HBP-A can effectively alleviate meniscal injuries induced by abnormal loading and suppress the expressions of genes related with pathological hypertrophy and calcification of the meniscus, and can serve as a potential drug for treatment of knee osteoarthritis.
