Effect of DNase I on biofilm formation of Staphylococcus aureus
- VernacularTitle:DNaseⅠ对金黄色葡萄球菌生物膜形成的影响
- Author:
Qiaoling XU
1
;
Fengjun SUN
;
Wei FENG
;
Xiao LIU
;
Yawei LIU
Author Information
1. 总参警卫局卫生保健处
- Keywords:
Staphylococcus aureus;
DNase I;
biofilm;
adhesion;
inhibition
- From:
Journal of Southern Medical University
2015;(9):1356-1359
- CountryChina
- Language:Chinese
-
Abstract:
Objective To study the effect of DNase I on biofilm formation of Staphylococcus aureus. Methods The growth curve of S. aureus was detected using a spectrophotometer. The adhesion of S. aureus was analyzed using flat colony counting method, and the biofilm formation was assayed using the 96-well crystal violet staining method. Results Exposure to different concentrations of DNase I did not obviously affect the growth of S. aureus but significantly inhibit the formation of bacterial biofilms in a dose-dependent manner. DNase I inhibited the adhesion of S. aureus at different growth stages. When combined with antibiotics, DNase I resulted in a significant decrease in the established biofilm biomass compared to antibiotics or DNase I used alone. Conclusion DNase I can effectively inhibit biofilm formation of S. aureus and enhance the inhibitory effect of antibiotics against S. aureus biofilms.
