Anti-miR-145 promotes human airway smooth muscle cell proliferation and osteopontin synthesis in vitro
10.3969/j.issn.1673-4254.2015.07.28
- VernacularTitle:Anti-miR-145促进人气道平滑肌细胞增殖及骨桥蛋白合成
- Author:
Peifen CHEN
1
;
Zhihui QIU
;
Guohua HUANG
;
Xiangmei ZHANG
;
Wujian PENG
;
Hui CENG
;
Wenyan LAI
Author Information
1. 广东深圳市第三人民医院内二科
- Keywords:
anti-miR-145;
airway smooth muscle cells;
proliferation;
osteopontin
- From:
Journal of Southern Medical University
2015;(7):1073-1075
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the effect of anti-miR-145 on human airway smooth muscle cell (HASMC) proliferation and osteopontin systhesis in vitro and explore the mechanisms. Methods HASMCs were treated with 10-100 nmol/L anti-miR-145, and the cell proliferation and apoptosis were investigated using a CCK-8 assay and flow cytometry, respectively. The changes in osteopontin synthesis after the treatment was quantified with Western blotting. Results Treatment with 10 and 50 nmol/L anti-miR-145 significantly promoted the proliferation and osteopontin synthesis in HASMCs (P<0.05 or<0.01), and 50 nmol/L anti-miR-145 obviously inhibited the cell apoptosis (P<0.01). Conclusion Anti-miR-145 promotes HASMC proliferation and osteopontin synthesis and inhibits HASMC apoptosis in vitro, indicating the important role of anti-miR-145 in the pathogenesis of airway remodeling.
