Sphingosine kinase 1 promotes glioma cell proliferation under hypoxia via calcium signaling
10.3969/j.issn.1673-4254.2015.07.15
- VernacularTitle:缺氧微环境下钙信号介导鞘氨醇激酶1促进胶质瘤细胞增殖
- Author:
He ZHANG
1
;
Linhua LIU
;
Ming SHI
;
Xiaoshan LIU
;
Huanwen TANG
Author Information
1. 广东医学院公共卫生学院劳动卫生与环境卫生学教研室
- Keywords:
sphingosine kinase 1;
hypoxia;
calcium signaling;
glioma
- From:
Journal of Southern Medical University
2015;(7):1014-1018
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the role of sphingosine kinase 1 (SphK1) in regulating the proliferation of hypoxia-exposed glioma cells in vitro and explore the possible molecular mechanisms. Methods Human glioblastoma U87MG cells was transfected with specific small interfering RNA (siRNA) constructs targeting SphK1, and the efficiency of SphK1 knockdown was validated by real-time PCR and Western blotting. The cells transfected with SphK1 siRNA and with a negative control siRNA were then exposed to 3%oxygen or 150μmol/L CoCl2 to induce hypoxia. The cell proliferation and cell cycle changes following the exposure were evaluated with the Cell Counting Kit-8 and flow cytometry, respectively, and the intracellular Ca2+changes were monitored using Flou-4/AM under an inverted laser scanning confocal microscope. Results SphK1 knockdown significantly reduced hypoxia-induced calcium reflux and suppressed the cell proliferation. Application of OAG, an activator of calcium channels, however, obviously enhanced the cell proliferation under hypoxia. Conclusion SphK1 promotes the proliferation of glioma cells under hypoxia via regulating calcium signaling.
