Effect of ouabain on intracellular Ca2+concentration in rat vascular smooth muscle cells in vitro
10.3969/j.issn.1673-4254.2015.07.06
- VernacularTitle:哇巴因对大鼠血管平滑肌细胞内钙离子浓度的影响
- Author:
Mingjuan ZHANG
1
;
Meicheng ZHANG
;
Chaoying ZHANG
;
Jun YANG
;
Canzhan ZHU
;
Zongming DUAN
Author Information
1. 西安交通大学医学院第二附属医院心内科
- Keywords:
sodium pump;
vascular smooth muscle cells;
ouabain;
Ca2+
- From:
Journal of Southern Medical University
2015;(7):960-965
- CountryChina
- Language:Chinese
-
Abstract:
Objective To explore the effect of ouabain on intracellular Ca2+ concentration ([Ca2+]i) in thoracic aorta vascular smooth muscle cells (VSMCs) in vitro. Methods Primary SD rat thoracic aorta VSMCs were cultured by tissue adherent method and identified by immunochemistry. The binding ability between ouabain and VSMCs was detected by autoradiography, and fluo 3-AM (a Ca2+fluorescent probe) was employed to investigate whether ouabain affected VSMCs within a short period of time. The effect of a truncated fragment of the sodium pumpα2 subunit was assayed in antagonizing the effect of ouabain on [Ca2+]i in the VSMCs. Results Within the concentration range of 0.1-100 nmol/L, ouabain was found to dose-dependently bind to the VSMCs. Different concentrations of ouabain (0-3200 nmol/L) caused a transient, dose-dependent increase in [Ca2+]i in the VSMCs, which was antagonized by the application of the truncated fragment of sodium pump α2 subunit. Conclusions Elevations in [Ca2+]i in the VSMCs can be the cytological basis of high ouabain-induced hypertension. The truncated fragment of the sodium pumpα2 subunit can antagonize ouabain-induced increase of [Ca2+]i in the VSMCs, which provides a clue for understanding the pathogenesis of and devising a therapeutic strategy for high ouabain-induced hypertension.
