Effect of RNA interference of CD59 gene on proliferation of non-small cell lung cancer cell line GLC-P in vitro
- VernacularTitle:RNA干扰抑制CD59对非小细胞肺癌GLC-P细胞株增殖的影响
- Author:
Weidong WU
1
;
Dan LIU
;
Wenjin HOU
;
Yongsheng YI
;
Yuejun WANG
;
Weijia LIN
Author Information
1. 广州市红十字会医院//暨南大学第四附属医院心胸外科
- Keywords:
RNA interference;
CD59;
non-small cell lung cancer;
GLC-P cells;
proliferation;
apoptosis
- From:
Journal of Southern Medical University
2015;(6):903-906
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the effect of CD59 gene inhibition mediated by RNA interference on the proliferation and apoptosis of non-small cell lung cancer (NSCLC) GLC-P cells in vitro. Methods Recombinant plasmids for RNA interference of CD59 gene were constructed and transfected into GLC-P cells via lipofectamine 2000. The stably transfected cells were examined with real-time RT-PCR, MTT assay and enzyme-linked immunosorbent assay to investigate the changes in cell proliferation and apoptosis. Results Compared with the control cells, the cells transfected with CD59-siRNA showed significantly decreased expression levels of CD59 mRNA (P<0.05) and significantly inhibited cell proliferation. Conclusions CD59 gene is highly expressed in NSCLC and RNA interference-mediated CD59 silencing can strongly inhibit the proliferation and induce apoptosis in GLC-P cells, which shed light on a potentially new target for targeted gene therapy of NSCLC.
