Compound K suppresses myeloid-derived suppressor cells in a mouse model bearing CT26 colorectal cancer xenograft
10.3969/j.issn.1673-4254.2015.05.25
- VernacularTitle:Compound K对小鼠CT26结肠肿瘤模型中髓系抑制细胞的抑制作用
- Author:
Rong WANG
1
;
Yalin LI
;
Wuzhou WANG
;
Meijuan ZHOU
;
Zhaohui CAO
Author Information
1. 南华大学 附属第一医院肿瘤内科
- Keywords:
ginseng-derived compound K;
myeloid-derived suppressor cells;
colonic tumors
- From:
Journal of Southern Medical University
2015;(5):748-752
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the effect of ginseng-derived compound K (C-K) on apoptosis, immunosuppressive activity, and pro-inflammatory cytokine production of myeloid-derived suppressor cells (MDSCs) from mice bearing colorectal cancer xenograft. Methods Flow-sorted bone marrow MDSCs from Balb/c mice bearing CT26 tumor xenograft were treated with either C-K or PBS for 96 h and examined for apoptosis with Annexin V/7-AAD, Cox-2 and Arg-1 expressions using qRT-PCR, and supernatant IL-1β, IL-6, and IL-17 levels with ELISA. C-K-or PBS-treated MDSCs were subcutaneously implanted along with CT26 tumor cells in WT Balb/c mice, and the tumor size and morphology were evaluated 21 days later. Results C-K treatment significantly increased the percentages of early and late apoptotic MDSCs in vitro (P<0.01 and P<0.05, respectively), decreased the expressions of immunosuppression-related genes Cox-2 (P<0.05) and Arg-1 (P<0.01), and suppressed the production of IL-1β (P<0.05), IL-6 (P<0.01), and IL-17 (P<0.05) by the MDSCs . Compared with PBS-pre-treated cells, C-K-pretreated MDSCs showed significantly attenuated activity in promoting CT26 tumor growth in mice (P<0.01). Conclusion C-K can suppress the immunosuppresive effect of MDSCs to inhibit tumor cell proliferation in mice, which suggests a new strategy of tumor therapy by targeting MDSCs.
