Ganoderic acid A suppresses proliferation and invasion and induces apoptosis in human osteosarcoma cells
10.3969/j.issn.1673-4254.2015.05.01
- VernacularTitle:灵芝酸A对人骨肉瘤细胞增殖、凋亡和迁移的影响
- Author:
Jianli SHAO
1
;
Zhizhong LI
;
Genlong JIAO
;
Guodong SUN
;
Zhigang ZHOU
Author Information
1. 暨南大学附属第一医院骨科
- Keywords:
ganoderic acid A;
human osteosarcoma cells;
proliferation;
apoptosis;
invasion
- From:
Journal of Southern Medical University
2015;(5):619-624
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the effect of ganoderic acid A (GA-A) on the biological behaviors of human osteosarcoma cells in vitro. Methods MG63 and HOS cells were treated with 0.1, 0.25, and 0.5 mmol/L GA-A, and the changes in cell proliferation, apoptosis and migration were evaluated using MTT assay, flow cytometry, and Transwell assay, respectively. The expressions of STAT3, p38, and NF-κB1 in the cells were analyzed by Western blotting. Results GA-A effectively inhibited the proliferation of human osteosarcoma HOS and MG-63 cells in a dose-dependent manner, and induced obvious cell apoptosis in both cells. Treatment with 0.5 mmol/L GA-A also resulted in significant inhibition of the invasion of both cells. The results of Western blotting showed that GA-A down-regulated the expression level of phosphorylated STAT3 and increased the phosphorylation level of p38 and NF-κB1 expression in both cells. Conclusion GA-A can induce proliferation inhibition, apoptosis and suppression of invasion in human osteosarcoma HOS and MG-63 cells.
