Human soluble dendritic cell- specific intercellular adhesion molecule 3- grabbing nonintegrin inhibits phagocytosis of Staphylococcus aureus by immature dendritic cells
- VernacularTitle:人可溶性DC-SIGN抑制未成熟树突状细胞吞噬金黄色葡萄球菌
- Author:
Huijie LI
1
;
Tianyu XU
;
Jia ZHOU
;
Lingyan ZHU
;
Liyun ZHANG
;
Xiao LU
;
Zhengliang CHEN
Author Information
1. 南方医科大学基础医学院免疫学教研室
- Keywords:
soluble dendritic cell- specific intercellular adhesion molecule 3-grabbing nonintegrin;
dendritic cells;
Staphylococcus aureus;
phagocytosis;
binding
- From:
Journal of Southern Medical University
2015;(4):544-548
- CountryChina
- Language:Chinese
-
Abstract:
Objective To study the effect and mechanism of soluble dendritic cell-specific intercellular adhesion molecule 3-grabbing nonintegrin (sDC-SIGN) on the phagocytosis of Staphylococcus aureus (S. aureus) by immature dendritic cells (imDCs). Methods Flow cytometry was employed to examine the effect of sDC-SIGN on the phagocytosis of S. aureus by imDCs. Enzyme-linked immunosorbent assay (ELISA) was used to analyze the binging of sDC-SIGN to S. aureus, lipoteichoic acid (LTA) and lipopolysaccharides (LPS) and investigate the effect of the ligands mannan and LTA and anti-DC-SIGN antibodies 1C6 and 4H3 on the binging of sDC-SIGN to S. aureus. Results sDC-SIGN inhibited the phagocytosis of S. aureus by imDCs. sDC-SIGN bound to S. aureus in a Ca2+-dependent manner. sDC-SIGN concentration-dependently bound to LTA, but not to LTA, and the binging of sDC-SIGN to S. aureus was blocked by mannan, LTA, 1C6 and 4H3. Conclusion sDC-SIGN preferentially binds to the carbohydrate constituents on S. aureus to affect the binding between membrane-bound DC-SIGN and S. aureus, thus suppressing the phagocytosis of S. aureus by imDCs.
