Application of 4-wavelength optical intrinsic signal imaging in monitoring peri-infarct depo-larizations in GFAP-/-Vim-/-mice
10.3969/j.issn.1673-4254.2015.03.21
- VernacularTitle:四波长内源光信号成像在GFAP-/-Vim-/-小鼠脑梗塞周围扩散性去极化中的应用
- Author:
Jianping L
1
;
Zhikai CAO
;
Jinmoo LEE
Author Information
1. 广州市第一人民医院神经外科
- Keywords:
brain infarction;
spreading depolarization;
optical intrinsic signal;
glial fibrillary acidic protein;
vimentin
- From:
Journal of Southern Medical University
2015;(3):417-421
- CountryChina
- Language:Chinese
-
Abstract:
Objective To study optical intrinsic signal (OIS) imaging of peri-infarct depolarizations (PIDs) in mice and investigate the influence of knockout of glial fibrillary acidic protein and vimentin on PIDs. Methods GFAP-/-Vim-/-mice and GFAP+/+Vim+/+mice were subjected to MCAO by standard intraluminal filament method. The main characteristics of PIDs in 4 h were studied by 4-wavelength OIS imaging technique. Results PIDs were identified as consistent, red and blue interaction waves in the cortical reflectance that slowly propagated peripherally from the origin site. There were 5 patterns of PID propagation, namely rostro-caudal, latero-medial, caudo-rostral, contralateral and medial-lateral. No significant differences were found in PID frequency, propagation patterns, velocity or duration time between the two groups (P>0.05). Conclusion The 4-wavelength OIS system allows acquisition of high temporal-spatial resolution color images for analyzing temporal-spatial characteristics of PIDs in detail. Knockout of GFAP and vimentin do not affect PIDs in 4 h following middle cerebral artery occlusion.
