Effect of IgG gene silencing by RNA interference on radiosensitivity of prostate cancer PC3 cells
10.3969/j.issn.1673-4254.2015.03.17
- VernacularTitle:RNA干扰IgG表达对人前列腺癌细胞株PC3放射敏感性的影响
- Author:
Yawen XU
1
;
Binshen CHEN
;
Kai XU
;
Bingkun LI
;
Yong WEN
;
Pengpeng ZHAO
Author Information
1. 南方医科大学珠江医院泌尿外科
- Keywords:
IgG;
RNA interference;
prostate cancer;
radiosensitivity
- From:
Journal of Southern Medical University
2015;(3):397-402
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the effect of RNA interference of IgG gene on the radiosensitivity of the human prostate cancer PC3 cell line. Methods PC3 cells were trasnfected via lipofectamine by the shRNA vector FCGR1AshRNA targeting the Fc segment of IgG, using NCshRNA as the negative control. Q-PCR and Western blotting were used to analyze the expression of IgG in the trasnfected cells. The cells were then exposed to 60Co γ ray at 0, 2, 4, 6, 8, 10 Gy, and the cell proliferation was evaluated by MTS and the cells apoptosis estimated by flow cytometry at 12, 24 and 48 h. Results MTS assay showed that 60Coγ ray significantly inhibited the proliferation of PC3 cells transfected with FCGR1AshRNA as compared with NCshRNA-transfected and blank control cells (P<0.05). Flow cytometry showed that the cell apoptosis rate was significantly higher in FCGR1AshRNA group than in NCshRNA and blank control groups at 48 h afterγray exposure (P<0.05). At 12, 24 and 48 h after 6 Gy radiation, the cells in FCGR1AshRNA group showed a significantly lowered proliferation rate and an increased apoptosis rate (P<0.05). Conclusion The shRNA targeting IgG gene can significantly enhance the sensitivity of PC3 cells to radiation. The combination of RNA interference targeting IgG gene with radiotherapy may be more effective in the treatment of prostate cancer.
