Adenovirus vector-mediated short hairpin RNA targeting nuclear factor-κB suppresses pro-liferation of endometrial cells of Macaca fascicularis in vitro
10.3969/j.issn.1673-4254.2015.03.16
- VernacularTitle:腺病毒介导靶向NF-κB基因的shRNA对体外食蟹猴子宫内膜细胞增殖活性的影响
- Author:
Fengcheng ZHU
1
;
Mubiao LIU
;
Ying PAN
;
Xuefeng WANG
;
Yanying CHEN
Author Information
1. 南方医科大学珠江医院妇产科
- Keywords:
endometriosis;
RNA interference;
nuclear factor Kappa B;
atadenovirus;
Macaca fascicularis
- From:
Journal of Southern Medical University
2015;(3):390-396
- CountryChina
- Language:Chinese
-
Abstract:
Objective To assess the effect of a high specific adenovirus vector-mediated shRNA targeting nuclear factor-κB (NF-κB) on cell proliferation of the endometrium of Macaca fascicularis. Methods The adenoviral vector NF-κB-p65-shRNA and the empty vector were separately trasnfected in cultured endometrial cells of Macaca fascicularis. The changes in the expression of the target gene protein and apoptotic proteins, cell proliferation, and cell cycle distribution were observed after the transfection. Results Compared with the control cells, infection of the endometrial cells with the NF-κB-p65-shRNA adenovirus significantly increased the expression levels of apoptotic proteins, promoted apoptosis of the endometrial cells, and reduced the cells in division stage. Conclusions NF-κB-p65 shRNA adenovirus can effectively promote apoptosis of endometrial cells and inhibit the proliferation of endometrial cells of Macaca fascicularis.
