Expression of connexin 43 in spinal cord dorsal horn of rats with acute incisional pain
10.3969/j.issn.1673-4254.2015.03.15
- VernacularTitle:急性切口痛对大鼠脊髓背角缝隙连接蛋白Cx43表达的影响
- Author:
Lingzhi WANG
1
;
Huansen HUANG
;
Zhikun WU
;
Zhengyuan XIA
;
Min LIAO
Author Information
1. 广州医科大学附属第二医院麻醉科
- Keywords:
incision pain;
gap junction;
connexin
- From:
Journal of Southern Medical University
2015;(3):387-389,396
- CountryChina
- Language:Chinese
-
Abstract:
Objective To examine the effect of acute incisional pain on the expression of connexin 43 in rat spinal cord dorsal horn. Methods Eighty rats were assigned into control group without any treatment and incisional pain group with incision surgery. For paw incisions, a 1-cm longitudinal incision was made through the skin and fascia of the plantar aspect of the right hind paw. After surgery, the 50% paw withdrawal threshold (PWT) was assessed in response to a tactile stimulus with calibrated von Frey monofilaments at 1, 2, 4 and 24 h, respectively. The spinal cord dorsal horn of rats was isolated at 1, 2, and 4 h after the surgery to assess the expression of connexin 43 using Western blotting and immunofluorescence assay. Results The 50%PWT of the rats was significantly decreased after the incision surgery, and this decrement was the most obvious at 2 and 4 h. Western blotting and immunofluorescence assay showed that the expression of connexin 43 in the spinal cord dorsal horn was significantly increased in rats receiving the surgery especially at 2 and 4 h after the surgery. Conclusion Incision surgery induces an significant increase in connexin 43 expression in rat spinal cord dorsal horn, suggestting an potential role of connexin43 in postoperative incisional pain.
