Expression of miR-223 in clear cell renal cell carcinoma and its significance
10.3969/j.issn.1673-4254.2015.03.05
- VernacularTitle:miR-223在肾透明细胞癌中的表达与功能
- Author:
Shaoxi NIU
1
;
Xin MA
;
Yu ZHANG
;
Huijie GONG
;
Yu GAO
;
Xintao LI
;
Donglai SHEN
;
Lei WANG
;
Yuanxin YAO
;
Xu ZHANG
Author Information
1. 中国人民解放军总医院泌尿外科//中国人民解放军总医院肾脏疾病国家重点实验室
- Keywords:
miR-223;
clear cell renal cell carcinoma;
migration;
proliferation
- From:
Journal of Southern Medical University
2015;(3):338-342
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the expression of miR-223 in clear cell renal cell carcinoma (ccRcc) and its clinical implications. Methods Quantitative real-time PCR was employed to detect the levels of miR- 223 expression in ccRcc, pair-matched adjacent normal tissues and different renal cancer cell lines. Transwell migration essay and wound healing essay were used to evaluate the invasion and migration of renal cancer 786-O cells transfected with miR-223 mimics. MTT essay was used to measure the cell proliferation, and the cell cycle changes following the transfection were analyzed with flow cytometry. Results Compared with the normal tissues, the cancer samples showed up-regulated miR-223 expression, which was associated with tumor size. In 786-O cell cultures, transfection with miR-223 mimics significantly enhanced cell migration (P<0.0001) and growth (P=0.006) and induced G1 cell cycle arrest. Conclusion miR-223 promotes renal cancer cell migration and proliferation and may serve as a potential therapeutic target for ccRcc.
