Intragastric administration of interferon- α- transformed Bifidobacterium promotes lymphocyte proliferation and maturation in mice
10.3969/j.issn.1673-4254.2015.03.03
- VernacularTitle:口服人α干扰素转化双歧杆菌促进小鼠淋巴细胞的增殖和成熟
- Author:
Weisen ZENG
1
;
Shuxian ZHANG
;
Congwen SHAO
;
Yuanjian HUANG
;
Wenying LIANG
;
Mingyue ZHENG
Author Information
1. 南方医科大学基础医学院细胞生物教研室
- Keywords:
bifidobacterium;
interferon-α2b;
lymphocytes;
proliferation;
maturation
- From:
Journal of Southern Medical University
2015;(3):326-332
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the effects of intragastric administration of human interferon-α (hIFN-α)-transformed Bifidobacterium on immune functions of mice. Methods The E.coli-Bifidobacterium shuttle expression vector containing hIFN-αgene was constructed and transformed into Bifidobacterium. The hIFN-α-transformed Bifidobacterium suspension (1010/ml) was prepared after induction with 0.2%L-arabinose for hIFN-αexpression and administered intragastrically in male Balb/C mice at the dose of 0.1 ml every other day for 2 weeks, with the mice receiving empty vector-transformed Bifidobacteria as the negative control and those having an equal volume of saline as the blank control. The percentages of mononuclear cell subsets in the thymus, spleen and blood were detected in the mice by flow cytometry, and the serum levels of IL-4, IL-12, IFN-γand TNF-αwere assayed using mouse cytokine FlowCytomix Kit. Results The percentages of CD3+CD8+and CD4+CD8+cells in the thymus, CD3+CD4+, CD3+CD8+and CD4+CD8+cells in the spleen, and CD3+CD8+cells in the blood all increased significantly in IFN group as compared with those in the negative and blank control groups (P<0.01 or 0.05). The serum level of IFN-γalso increased significantly (P<0.05) while IL-4 level remained unchanged in IFN group compared with those in the two groups. Conclusion Intragastric administration of hIFN- α- transformed Bifidobacterium promotes lymphocyte proliferation and maturation and increases the serum levels of Th1 cytokines in mice.
