Src kinase inhibitor PP2 protects rat astrocytes from hypoxia/reoxygenation injury in vitro
10.3969/j.issn.1673-4254.2015.02.16
- VernacularTitle:Src激酶抑制剂PP2对星形胶质细胞缺氧/复氧损伤的保护作用
- Author:
Yuchen GU
1
;
Xuhui TONG
;
Li YU
;
Hao JIAO
;
Binbin YU
;
Shuying DONG
Author Information
1. 蚌埠医学院药学系药理学教研室
- Keywords:
astrocytes;
hypoxia/reoxygenation;
Src kinase;
PP2
- From:
Journal of Southern Medical University
2015;(2):239-243
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the effect of Src kinase inhibitor PP2 on hypoxia/reoxygenation (H/R) injury in rat astrocytes in vitro. Methods In vitro cultured rat astrocytes were exposed to hypoxia for 8 h followed by reoxygenation for 24 h with or without pretreatment with PP2 (10 μmol/L) for 24 h before H/R injury. MTT assay and flow cytometry were used to detect the viability and apoptosis of the exposed astrocytes, respectively, and the protein expressions of Src, Bax, and Bcl-2 in the cells were determined using Western blotting. Results PP2 pretreatment significantly increased the viability and decreased the apoptosis rate of rat astrocytes exposed to H/R injury (P<0.01). Western blotting showed that H/R injury caused increased expression of Src kinase, which was lowered by PP2 pretreatment. The ratio of Bax/bcl-2 in the astrocytes increased after H/R injury, and was significantly decreased by PP2 pretreatment (P<0.01). Conclusion PP2 protects rat astrocytes from H/R injury possibly by inhibiting the expression of Src kinase and activating the anti-apoptotic mechanisms in the cells.
