Differential expression of miRNAs in myocardial tissues of rats with lipopolysaccharide-in-duced endotoxemia
10.3969/j.issn.1673-4254.2015.02.11
- VernacularTitle:内毒素血症大鼠心肌组织miRNA差异表达
- Author:
Tao DING
1
;
Yang LI
;
Ruizhu TANG
;
Xuan ZHANG
;
Yu YUN
;
Jian LI
;
Dianhua WANG
Author Information
1. 昆明医科大学药学院
- Keywords:
endotoxemia;
lipopolysaccharide;
myocardial injury;
miRNA;
expression profile
- From:
Journal of Southern Medical University
2015;(2):213-217
- CountryChina
- Language:Chinese
-
Abstract:
Objective To analyze the differential miRNA expression profile in the myocardium of rats with lipopolysaccharide (LPS)-induced endotoxemia and explore the role of miRNA in endotoxin-induced myocardial injury. Methods Twenty male SD rats received intraperitoneal injection of 10 mg/kg LPS (n=10) or an equivalent amount of saline solution (n=10). At 24 h after LPS injection, the rats were sacrificed to detect myocardial expressions of TLR4, TNF-αand IL-1βusing real-time PCR and for observing myocardial ultrastructures under transmission electron microscopy. The differentially expressed miRNA in the myocardium were detected using a miRNA array, and the common differentially expressed miRNAs were selected for verifying their actual expressions using real-time PCR. Results TLR4, TNF-αand IL-1βwere over-activated in the myocardium of LPS-treated rats, in which mitochondria swelling, structural damaged and cytoplasmic vacuoles were observed. In LPS-challenged rats, miR-194-3p, miR-344a-3p, miR-465-3p, miR-501-5p, miR-3596c, miR-185-3p, and miR-877 were found up-regulated significantly, whereas miR-208b-3p, miR-547-3p, miR-141-3p, miR-28-5p, and miR-3585-5p down-regulated in the myocardium. Conclusion Significant differential expression of the miRNAs occurs in the myocardium of LPS-treated rats, suggesting their involvement in endotoxin-induced myocardial injury.
