Effect of repeated hypoxic preconditioning on renal ischemia-reperfusion-induced hepatic dysfunction in rats
10.3969/j.issn.1673-4254.2015.01.31
- VernacularTitle:重复性低氧预处理对大鼠肾缺血再灌注所致肝脏损伤的影响
- Author:
Na YAN
1
,
2
;
Zeguo FENG
;
Guangtao YAN
;
Jianhong YUE
;
Yanjun ZHAO
;
Na GENG
Author Information
1. 解放军总医院 麻醉手术中心
2. 吉林大学第一医院
- Keywords:
repetitive hypoxic preconditioning;
nephrology;
ischemia;
reperfusion injury;
hepatic dysfunction
- From:
Journal of Southern Medical University
2015;(1):149-153
- CountryChina
- Language:Chinese
-
Abstract:
Objective To explore the effect of repeated hypoxic preconditioning (RHP) on renal ischemia-reperfusion-induced hepatic dysfunction in rats and the underlying mechanism. Methods A total of 120 normal SD rats were randomly divided into 4 groups (n=40), namely RHP surgical group, RHP sham-operated (RHPS) group, nonhypoxic surgical group (IRI group), and nonhypoxic sham-operated group (S group). The rats in the hypoxic groups were exposed to hypoxia in a hypoxic chamber for 5 days prior to establishment of renal ischemia-reperfusion model by resection of the right kidney and clamping the left renal hilum. Serum alanine aminotransferase (ALT), IL-17A, TNF-α, liver superoxide dismutase (SOD) and nitric oxide (NO) were detected at 2, 8 and 24h after reperfusion, and Western blotting was used to determine the expression of p-PI3K and p-AKT;HE staining was used to observe the structural changes in the liver. Results Compared with IRI group, RHP group showed significantly milder hepatic damage, lower ALT levels and higher NO levels at 2, 8, and 24 after reperfusion (P<0.05);TNF-αlevels were lowered at 24 h (P<0.05) and SOD increased at 8 h after the reperfusion (P<0.05). Compared with S group, IRI group and RHP group showed significantly higher IL-17A levels (P<0.05) but without significant difference between the latter two groups (P>0.05). The expressions of p-PI3K and P-Akt in RHP group were significantly higher than those in IRI group (P<0.05), especially at 8 h after reperfusion (P<0.05). Conclusion Repeated hypoxic preconditioning can attenuate hepatic injury induced by renal ischemia-reperfusion injury in rats.
