Establishment of a cell model of insulin-resistant 3T3-L1 adipocytes
10.3969/j.issn.1673-4254.2015.01.20
- VernacularTitle:IR-3T3-L1脂肪胰岛素抵抗细胞的建立
- Author:
Xuqiang NIE
1
;
Jianwen YANG
;
Haixia SHI
;
Yujin ZHANG
;
Jianyong ZHANG
;
Ka BIAN
Author Information
1. 遵义医学院药学院
- Keywords:
3T3-L1 adipocytes;
insulin resistance;
dexamethason;
glucose consumption;
insulin
- From:
Journal of Southern Medical University
2015;(1):103-108
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the optimal conditions for establishing insulin-resistant 3T3-L1 adipocytes. Methods Dexamethason (DEX), 3-isobutyl-methylxanthine (IBMX) and different concentrations of insulin (10-8, 10-7, and 10-6 mol · L-1) were used to induce 3T3-L1 preadipocytes into mature adipocytes identified by oil red O staining. We established insulin-resistant 3T3-L1 adipocytes cell model (IR-3T3-L1) by exposing the cells to 1μmol· L-1 DEX, and the changes of glucose concen-tration in the cell culture were determined by glucose oxidase-peroxidase (GOD-POD) assay. Results Treatment of 3T3-L1 cells with DEX, IBMX and 10-6 mol· L-1 insulin for 9 days resulted in the differentiation of>90%of the cells into mature adipocytes. IR-3T3-L1 cells cultured for 96 h in the culture media containing 1 μmol · L-1 DEX showed significantly increased glucose consumption (P=0.0003) as compared with the control group at 36 h (P<0.001). Conclusion 3T3-L1 cells can be induced into mature adipocytes by exposure to 1μmol· L-1 DEX, 0.5 mmol· L-1 IBMX and 10-6 mol· L-1 insulin. A 96 h exposure to 1μmol· L-1 DEX can induce 3T3-L1 adipocytes to acquire insulin resistance that can be maintained for 36 h.
